Mechanism of modulation of the plasma membrane Ca(2+)-ATPase by arachidonic acid

Prostaglandins & Other Lipid Mediators
Vanessa H OliveiraJulio A Mignaco

Abstract

The intracellular level of long chain fatty acids controls the Ca(2+) concentration in the cytoplasm. The molecular mechanisms underlying this Ca(2+) mobilization are not fully understood. We show here that the addition of low micromolar concentrations of fatty acids directly to the purified plasma membrane Ca(2+)-ATPase enhance ATP hydrolysis, while higher concentration decrease activity, exerting a dual effect on the enzyme. The effect of arachidonic acid is similar in the presence or absence of calmodulin, acidic phospholipids or ATP at the regulatory site, thereby precluding these sites as probable acid binding sites. At low arachidonic acid concentrations, neither the affinity for calcium nor the phosphoenzyme levels are significantly modified, while at higher concentrations both are decreased. The action of arachidonic acid is isoenzyme specific. The increase on ATP hydrolysis, however, is uncoupled from calcium transport, because arachidonic acid increases the permeability of erythrocyte membranes to calcium. Oleic acid has no effect on membrane permeability while linoleic acid shows an effect similar to that of arachidonic acid. Such effects might contribute to the entry of extracellular Ca(2+) following to fatty acid r...Continue Reading

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Citations

Mar 2, 2011·Pflügers Archiv : European journal of physiology·Mauricio A RetamalLuis Reuss
Aug 11, 2015·Ukrainian biochemical journal·T O VeklichS O Kosterin
May 24, 2013·International Journal of Molecular Sciences·Anna BogdanovaLars Kaestner
Jun 3, 2021·International Journal of Molecular Sciences·Stefano TuroloGiovanni Montini

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