Mechanism of PAF-induced platelet aggregation in man

Thrombosis Research
L J KüsterJ C Frölich

Abstract

The present study was designed to investigate the mechanisms involved in aggregation induced by platelet-activating factor (PAF) in human platelet-rich plasma (PRP). PAF induced dose-dependent aggregation over the range of 50 nM to 14 microM, with a threshold dose of about 100 nM. BN 52021, a recently described PAF antagonist, completely abolished the effect of PAF at a ten-fold higher concentration. None of the concentrations of PAF used significantly increased TXB2 release. In plasma obtained from volunteers who had taken 500 mg acetylsalicylic acid over five days, no change of PAF-induced aggregation could be observed in comparison to the control state. The lipoxygenase inhibitors nordihydroguaiaretic acid and BW 755 C also failed to significantly modify the PAF-induced platelet response. Pretreatment of PRP with the calcium channel blockers verapamil and nifedipine and the calmodulin antagonist trifluoperazine inhibited platelet aggregation by PAF over the entire range tested. These data indicate that PAF may utilize a specific membrane receptor, which can be blocked by BN 52021. Its aggregatory effect is probably mediated via the calcium-calmodulin system. Moreover, derivatives of arachidonic acid do not appear to be prima...Continue Reading

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Citations

Mar 15, 1987·Thrombosis Research·J Filep, E Földes-Filep
Jan 1, 1989·Comparative Biochemistry and Physiology. A, Comparative Physiology·P A GentryR M Liptrap
Sep 1, 1992·Comparative Biochemistry and Physiology. Comparative Physiology·M A PackhamR L Kinlough-Rathbone
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Jan 1, 1992·British Journal of Clinical Pharmacology·M L TaylorP J Thompson
Jan 1, 1989·Thrombosis Research·L J Küster, J C Frölich
Feb 15, 1991·Thrombosis Research·J G FilepE Földes-Filep
Oct 15, 1987·Thrombosis Research·F VioliC Cordova
May 1, 1989·Stroke; a Journal of Cerebral Circulation·R JosephG D'Andrea
Sep 22, 2020·Natural Products and Bioprospecting·Tin Thu Thu AungYue-Hu Wang
Aug 4, 2018·Natural Products and Bioprospecting·Meng-Yuan XiaYue-Hu Wang
Apr 13, 2019·Natural Products and Bioprospecting·Hou-Chao XuPema-Tenzin Puno
Oct 1, 1992·Journal of Comparative Pathology·P A Gentry

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