Mechanism of Pdia3-dependent 1α,25-dihydroxy vitamin D3 signaling in musculoskeletal cells

Steroids
B D BoyanZ Schwartz

Abstract

1α,25-Dihydroxy vitamin D3 [1,25(OH)2D3] acts on cells through traditional steroid hormone receptor-mediated gene transcription and by initiating rapid membrane-associated signaling pathways. Two receptors have been implicated in rapid signaling by 1,25(OH)2D3, the classical nuclear vitamin D receptor (VDR) and the more recently identified protein disulfide isomerase, family A, member 3 (Pdia3). Our lab along with other groups has established various tools to investigate the role of these two receptors, including gene knock-out, conditional knock-out, silencing, and over-expression in various model systems (growth plate chondrocytes, osteoblastic cells, chick intestinal epithelial cells, mouse embryoid bodies, extracellular matrix vesicles and isolated cell membranes). The data demonstrate the requirement for Pdia3 in 1,25(OH)2D3 induced phospholipase A2 (PLA2) and protein kinase C (PKC) activation and downstream responses. Pdia3+/- heterozygote mice also exhibit both cartilage and bone defects. VDR is present on the plasma membrane and one VDR-/- mouse strain lacks transcaltachia, although 1,25(OH)2D3 induced PKC activation and transcaltachia are not affected in another VDR-/- mouse strain. In the context of osteoblast differe...Continue Reading

References

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Citations

Nov 21, 2013·Steroids·Reyhaan A ChaudhriBarbara D Boyan
Jul 23, 2013·Journal of Neurology, Neurosurgery, and Psychiatry·Rui LinIngrid A F van der Mei
Jan 21, 2014·Cellular and Molecular Neurobiology·Gabriela P de OliveiraGerson Chadi
Dec 21, 2012·Molecular Carcinogenesis·Antoine MénoretDaniel W Rosenberg
Dec 24, 2014·BMC Genomics·Marine GueugneauDaniel Béchet
Nov 19, 2014·PloS One·Yun WangBarbara D Boyan
Jun 12, 2016·Biochimica Et Biophysica Acta·Nofrat SchwartzBarbara D Boyan
Nov 30, 2016·Scientific Reports·Elisa GaucciSilvia Chichiarelli
Jan 29, 2017·The Journal of Steroid Biochemistry and Molecular Biology·Kristina M DiFrancoGill Diamond
Feb 18, 2017·Nutrients·Lucinda J BlackJanet F Bornman
Jul 21, 2017·Pharmacology & Therapeutics·Aubryanna HettinghouseChuan-Ju Liu

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