Mechanism of PKA-dependent and lipid-raft independent stimulation of Connexin43 expression by oxytoxin in mouse embryonic stem cells.

Molecular Endocrinology
Seung Pil YunHo Jae Han

Abstract

Previous studies shows that connexins appear very early during murine embryo development, the gap junctional intercellular communication found in the inner cell mass of early embryo is also maintained in embryonic stem cells (ESC), and expression of oxytocin receptor (OTR) is developmentally regulated at early embryonic development. However, effect of oxytocin (OT) on the regulation of the connexin43 (Cx43) and maintenance of undifferentiation is not fully understood in stem cells. Therefore, we investigated the effect of OT on Cx43 expression and related signaling cascades in mouse ESC. OT increased Cx43 expression that was inhibited by the OTR inhibitor atosiban. In experiments to examine whether the effect of OT depends on lipid rafts, caveolin-1 (cav-1), cav-2, and flotillin-2, but not OTR, were detected in lipid raft fractions. Also, colocalization of OTR, cav-1, and cav-2 was not detected. Moreover, the lipid raft disruptor methyl-β-cyclodextrin did not attenuate OT-induced Cx43 expression. In experiments to examine related signaling pathways, OT activated cAMP/protein kinase A (PKA) which was inhibited by adenylyl cyclase inhibitor SQ 22536 and PKA inhibitor PKI. OT increased nuclear factor κ-light-chain-enhancer of acti...Continue Reading

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Citations

Mar 21, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Michael K G StewartDale W Laird
Jul 17, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·R B GilchristJ G Thompson
Feb 9, 2017·Journal of Applied Toxicology : JAT·Qingping LiuQing Wu
Feb 22, 2017·The Journal of Endocrinology·Hai-Fan YuBin Guo
Mar 6, 2018·The Journal of Reproduction and Development·Satoshi SugimuraRobert B Gilchrist
Sep 14, 2019·Endocrinology·Masahide YoshidaKatsuhiko Nishimori
Jan 1, 2021·Cancers·Chang-Xu ChenJean X Jiang

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