Mechanism of polyamine toxicity in cultured cardiac myocytes

Toxicology in Vitro : an International Journal Published in Association with BIBRA
U R Tipnis, G Y He

Abstract

The goal of this study was to investigate the mechanism of polyamine-mediated injury to the cardiac myocytes isolated from neonatal rat hearts. The myocytes, cultured in Dulbecco's minimal essential medium-1% foetal calf serum (FBS), were exposed to spermidine or spermine. The toxicity to myocytes was determined by (a) increased release of creatine kinase (CPK) into the media and (b) decline in cell viability or functional activity. Spermidine, above 10 mum, increased the release of CPK into media, decreased cell viability and decreased the functional activity of the myocytes. The FBS exhibited polyamine oxidase activity and semicarbazide-sensitive amine oxidase activity. Aminoguanidine, MDL72,527 or semicarbazide, are the inhibitors of amine oxidases, polyamine oxidase (PAO) and semicarbazide-sensitive amine oxidase (SSAO), respectively. The addition of these inhibitors to the medium protected the myocytes from spermidine toxicity. To determine whether myocyte PAO is involved in polyamine toxicity, we used horse serum that contained high SSAO activity and negligible PAO activity. The myocyte extracts had negligible SSAO activity but high PAO activity. When myocytes were cultured in horse serum in lieu of FBS, spermine caused t...Continue Reading

References

Apr 6, 1978·Biochimica Et Biophysica Acta·J JänneA Raina
Aug 1, 1991·Annals of Medicine·J JänneP Leinonen
Jan 15, 1991·Biochemical Pharmacology·M B Youdim, J P Finberg
Mar 1, 1990·Differentiation; Research in Biological Diversity·R A GramzinskiG B Pierce
Sep 1, 1982·Archives of Biochemistry and Biophysics·H Pösö, A E Pegg
Sep 15, 1994·Biochemical Pharmacology·E AgostinelliD A Averill-Bates
Jul 14, 1994·Regulatory Peptides·U R TipnisJ Gutkowska

❮ Previous
Next ❯

Citations

Nov 7, 2000·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·U R TipnisP J Boor
Oct 31, 2009·Journal of Enzyme Inhibition and Medicinal Chemistry·Sylvain LozanoSylvain Rault

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.