Mechanism of programmed cell death factor 4/nuclear factor-κB signaling pathway in porcine coronary micro-embolization-induced cardiac dysfunction

Experimental Biology and Medicine
Qiang SuYangchun Liu

Abstract

The aim of this study was to investigate the role of the programmed cell death factor 4 (PDCD4)/nuclear factor-κB (NF-κB) signaling pathway in coronary micro-embolism (CME)-induced inflammatory responses and cardiac dysfunction in a porcine model. Bama miniature pigs were randomly divided into four groups (n = 5 per group). Micro-embolization balls or saline were infused through a microcatheter in the left anterior descending (LAD) artery in the CME and Sham groups, respectively. PDCD4 siRNA or control siRNA mixed with transfection reagent was infused via the LAD artery 72 h before CME induction in the CME + siRNA-PDCD4 and siRNA-control groups, respectively. Cardiac function was evaluated with ultrasound. Tissue biopsy was stained with hematoxylin-eosin (HE) and hematoxylin basic fuchsin picric acid (HBFP) to measure infarction area. Myocardial PDCD4 and tumor necrosis factor-α (TNF-α) mRNA and protein expression were analyzed by quantitative PCR and Western blotting. NF-κB activity was evaluated in gel electrophoretic mobility shift assay. Echocardiographic parameters showed that compared with the sham group, the CME group had impaired heart function, manifested as systolic dysfunction and left ventricular dilatation (reduced...Continue Reading

References

Jan 29, 2002·Journal of Molecular and Cellular Cardiology·Hilmar DörgeGerd Heusch
Nov 23, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anja HilliardYouhai H Chen
Aug 27, 2009·The Journal of Biological Chemistry·Shimin DongChunxiang Zhang
Nov 4, 2009·Circulation·Gerd HeuschRaimund Erbel
Jul 12, 2014·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jiangyou WangYangchun Liu
Aug 15, 2014·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Qiang SuGuotian Ma

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Methods Mentioned

BETA
transfection
electrophoretic mobility shift assay
PCR
electrophoresis
X-ray
gene knockout

Software Mentioned

Leica Qwin
SPSS16

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