Mechanism of programmed obesity in intrauterine fetal growth restricted offspring: paradoxically enhanced appetite stimulation in fed and fasting states.

Reproductive Sciences
Tatsuya FukamiMichael G Ross

Abstract

We have shown that intrauterine fetal growth restriction (IUGR) newborn rats exhibit hyperphagia, reduced satiety, and adult obesity. Adenosine monophosphate (AMP)-activated protein kinase (AMPK) is a principal metabolic regulator that specifically regulates appetite in the hypothalamic arcuate nucleus (ARC). In response to fasting, upregulated AMPK activity increases the expression of orexigenic (neuropeptide Y [NPY] and agouti-related protein [AgRP]) and decreases anorexigenic (proopiomelanocortin [POMC]) peptides. We hypothesized that IUGR offspring would exhibit upregulated hypothalamic AMPK, contributing to hyperphagia and obesity. We determined AMPK activity and appetite-modulating peptides (NPY and POMC) during fasting and fed conditions in the ARC of adult IUGR and control females. Pregnant rats were fed ad libitum diet (control) or were 50% food restricted from gestation day 10 to 21 to produce IUGR newborns. At 10 months of age, hypothalamic ARC was dissected from fasted (48 hours) and fed control and IUGR females. Arcuate nucleus messenger RNA ([mRNA] NPY, AgRP, and POMC) and protein expression (total and phosphorylated AMPK, Akt) was determined by quantitative reverse transcriptase-polymerase chain reaction and West...Continue Reading

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Citations

Jun 25, 2013·Trends in Endocrinology and Metabolism : TEM·Daniella E Duque-Guimarães, Susan E Ozanne
Jul 24, 2014·Journal of Developmental Origins of Health and Disease·T FukamiM G Ross
Nov 21, 2015·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Lian-Hui ChenWei-Fen Zhu
Mar 26, 2016·Frontiers in Physiology·Amanda N Sferruzzi-Perri, Emily J Camm
Sep 29, 2015·Journal of Developmental Origins of Health and Disease·R Dalle MolleP P Silveira
Mar 22, 2014·Annals of the New York Academy of Sciences·André Krumel Portella, Patrícia Pelufo Silveira
Oct 31, 2018·International Journal of Molecular Sciences·Jasmine F PlowsMark H Vickers
Oct 12, 2018·International Journal of Molecular Sciences·Asako KumagaiKeizo Kanasaki

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Methods Mentioned

BETA
PCR
Immuno
Precipitation
Assay
enzyme-linked immunosorbent assay

Software Mentioned

Image J

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