PMID: 6383865Oct 1, 1984

Mechanism of protein biosynthesis in prokaryotic cells. Effect of initiation factor IF1 on the initial rate of 30 S initiation complex formation

FEBS Letters
C L Pon, Claudio O Gualerzi

Abstract

To define the step at which translational initiation factor IF1 exercises its stimulation, initial rate kinetic analyses of 30 S initiation complex formation were carried out in the presence and absence of this factor. It was shown that, without affecting the affinity of the ribosomes either for the initiator tRNA or for the poly(AUG) used as template, IF1 increases approximately 2.5-fold the limiting Vmax of the 'pre-ternary complex'----ternary complex transition which represents the rate-limiting step in 30 S initiation complex formation. This kinetic effect titrates with the 30 S ribosomal subunit which must therefore represent the target of IF1 action.

References

Aug 23, 1972·Nature: New Biology·M Noll, H Noll
May 10, 1972·Biochimica Et Biophysica Acta·R BenneH O Voorma
Jan 15, 1973·European Journal of Biochemistry·R BenneH O Voorma
Jun 1, 1974·Archives of Biochemistry and Biophysics·H F KungH Weissbach
Oct 15, 1971·FEBS Letters·Rajarshi Mazumder

Citations

Dec 15, 1988·European Journal of Biochemistry·B CelanoClaudio O Gualerzi
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Aymen S YassinRajendra K Agrawal
May 26, 2006·The EMBO Journal·Ayman AntounMåns Ehrenberg
Mar 27, 2007·Journal of Bacteriology·Sangita PhadtareKonstantin Severinov
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Brian Søgaard LaursenHans Uffe Sperling-Petersen
Feb 6, 2010·Molecular Microbiology·Anna ZorzetDan I Andersson
Feb 19, 2016·Proceedings of the National Academy of Sciences of the United States of America·Hiroshi YamamotoKnud H Nierhaus
Jan 11, 2019·International Journal of Molecular Sciences·Jing ZhaoGong Zhang
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·C O GualerziC L Pon
Jan 20, 2004·European Journal of Biochemistry·Victor V CroitoruLeif A Isaksson
Oct 21, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Sangita Phadtare, Konstantin Severinov

Related Concepts

Alkalescens-Dispar Group
Peptide Chain Initiation, Translational
Peptide Initiation Factors
Ribosomes
Genetic Template
Protein Biosynthesis
Eukaryotic Initiation Factor-1

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