PMID: 2496757Feb 7, 1989Paper

Mechanism of protein-induced membrane fusion: fusion of phospholipid vesicles by clathrin associated with its membrane binding and conformational change

Biochemistry
S MaezawaD Papahadjopoulos

Abstract

The clathrin-induced fusion of liposome membranes, the membrane binding of clathrin, and the conformational states of clathrin were investigated over a wide pH range using large unilamellar and multilamellar vesicles composed of phosphatidylserine (PS), phosphatidylcholine (PC), PS/PC (2:1), PS/PC (1:1), or PS/PC (1:2). The pH profiles of clathrin-induced fusion of all types of liposomes containing PS showed biphasic patterns. Their pH thresholds were found in the pH range of 5-6 and shifted to lower pH values with decrease in the PS content. Similar shifts were observed in the pH range of 5-6 and shifted to lower pH values with decrease in the PS content. Similar shifts were observed in the pH profiles of clathrin binding to these vesicles, but the pH profiles of binding were different from the biphasic fusion patterns. With PC vesicles, only small degrees of fusion and clathrin binding were observed at pH 2-4. The pH dependences of the conformation and hydrophobicity of clathrin were determined by measuring the extent of the blue shift of the fluorescence maximum of 1-anilinonaphthalene-8-sulfonate in the presence of the protein, the fluorescence intensity of N-(1-anilinonaphthyl-4)maleimide bound to the clathrin molecule, th...Continue Reading

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Citations

Feb 20, 1992·Biochimica Et Biophysica Acta·K ArnoldS Ohki
Mar 3, 2010·Annual Review of Biophysics·Peter A Leventis, Sergio Grinstein
Jun 18, 1990·FEBS Letters·V K LishkoI O Trikash
Dec 9, 1991·Biochimica Et Biophysica Acta·S Maezawa, T Yoshimura
Nov 30, 1990·Biochemical and Biophysical Research Communications·S Maezawa, T Yoshimura
Jul 5, 2012·PloS One·Herre Jelger RisseladaMarcus Müller
May 5, 2017·International Journal of Molecular Sciences·Uyen Thi Trang ThanTony Parker
Jan 1, 1994·Critical Reviews in Biochemistry and Molecular Biology·A Sánchez-FerrerF García-Carmona
Apr 13, 1990·Biochimica Et Biophysica Acta·J P PrivatM Ptak

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