Mechanism of regulating the expression of lambdaN gene by ribosomal protein at translational level

Science in China. Series C, Life Sciences
Muyang LiK Tong

Abstract

In ribosomal protein S12 mutant or L24 mutant the expression of lambdaN gene was depressed at translational level. To study its mechanism the lambdaN gene region of lambdaN -lacZ gene fusion was trimmed from its 5' end to 3' end with DNA exonuclease III (DNA exoIII) in order to alter the TIR (translational initiation region) and the coding region of lambdaN gene. After DNA sequencing 23 species of different lambdaN-lacZ fused genes were obtained. The beta-galactosidase activities of these deletants in ribosomal protein mutant were compared with that in wild type strain. The result indicated that (i) S12 mutant could affect 305 subunit's binding to the TIR of lambdaN gene messenger and cause the difficulty in forming 30s initiation complex and then decrease the efficiency of translational initiation; (ii) in S12 mutant the coding region of lambdaN gene also affected the expression lambdaN gene; (iii) in L24 mutant the inhibition of lambdaN gene expression was not related to translational initiation and the 5' end of the coding region of lambdaN gene, but related to the 3' end of lambdaN gene.

References

Jan 5, 1992·Journal of Molecular Biology·J A Gallant, D Lindsley
Mar 1, 1990·Trends in Genetics : TIG·J E McCarthy, C Gualerzi
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M H de Smit, J van Duin
May 20, 1989·Journal of Molecular Biology·M A SørensenS Pedersen
Feb 25, 1995·Nucleic Acids Research·J F Curran
May 5, 1995·Journal of Molecular Biology·H Dong, C G Kurland
Jan 7, 1994·Journal of Molecular Biology·M H de Smit, J van Duin

❮ Previous
Next ❯

Citations

Mar 29, 2013·PloS One·João Alves GamaFrancisco Dionisio

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.