Mechanism of repression of squamous differentiation marker, SPRR1B, in malignant bronchial epithelial cells: role of critical TRE-sites and its transacting factors

Oncogene
T PattersonS P Reddy

Abstract

The overexpression of SPRR1B in bronchial epithelium is a marker for early metaplastic changes and the loss of its expression is associated with an irreversible malignant transformation. In the present study, we have used a model system consisting of normal and malignant bronchial epithelial (BE) cells to elucidate the differential transcriptional control of SPRR1B. SPRR1B expression is either detectable or PMA (phorbol 13-myristate 12-acetate) -inducible in several malignant BE cells including squamous, adeno, small and large cell carcinomas. Loss of SPRR1B expression is correlated well with the lack of strong in vivo protein-DNA interactions at the -152 bp promoter, which contains two functional TRE sites. Even though the basal level AP-1 protein DNA binding pattern is different between normal and malignant cells, PMA significantly enhances Jun and Fos binding to the consensus TRE site in both cell types. Intriguingly, the composition of AP-1 protein binding to the -152 to -86 bp SPRR1B promoter is quite different. In untreated cells, SPRR1B promoter is predominantly occupied by JunD and Fra2. PMA significantly induced binding of JunB and Fra1 in normal cells, while JunB and Fra2 bound to TREs in the malignant cells. Overexpr...Continue Reading

References

May 11, 1992·Molecular Endocrinology·T M VollbergA M Jetten
Sep 1, 1991·Molecular and Cellular Biology·K Kovary, R Bravo
Oct 25, 1991·Nucleic Acids Research·T SuzukiH Iba
Aug 1, 1990·The American Journal of Physiology·C Basbaum, B Jany
Apr 1, 1995·Experimental Cell Research·M YaarB A Gilchrest
Mar 1, 1994·The Journal of Investigative Dermatology·M McClaren, R R Isseroff
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C AbateT Curran
Jan 1, 1995·Pediatric Radiology·O R Tenreiro-PiconK Y Lau
Jan 1, 1996·Molecular Biology Reports·R L Eckert, J F Welter
Apr 1, 1997·Current Opinion in Cell Biology·M KarinE Zandi
Jul 3, 1998·American Journal of Respiratory Cell and Molecular Biology·J P DeMuthJ C Willey
Aug 12, 1998·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·R HuD Lau
Jun 5, 1999·Cell Biochemistry and Biophysics·J Tesfaigzi, D M Carlson
Jun 12, 1999·Differentiation; Research in Biological Diversity·D DiSepioD R Roop
Dec 14, 1999·The Journal of Thoracic and Cardiovascular Surgery·K M FongJ D Minna
Dec 30, 1999·American Journal of Respiratory Cell and Molecular Biology·D LauS Reddy
Mar 29, 2000·Journal of Cellular Physiology·R L Momparler, V Bovenzi
Jun 8, 2000·International Journal of Cancer. Journal International Du Cancer·U SotoF Rösl
Aug 3, 2000·Journal of the National Cancer Institute·P A MarksR A Rifkind

❮ Previous
Next ❯

Citations

Jul 2, 2002·American Journal of Respiratory Cell and Molecular Biology·Sekhar P M ReddyHue Vuong
Jan 23, 2009·Cancer Investigation·Kai MaTianyou Wang
Mar 15, 2008·Pulmonary Pharmacology & Therapeutics·Charles G Plopper, Dallas M Hyde
May 10, 2017·International Journal of Cancer. Journal International Du Cancer·Keqiang ZhangDan J Raz
Nov 7, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·James N McDougalSteven J Berberich
Nov 9, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sekhar P M Reddy, Brooke T Mossman
Mar 27, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jacqui M MarzecSteven R Kleeberger
Mar 29, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alena PanceJean-François Jeannin
Oct 21, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Qin ZhangSekhar P Reddy
Nov 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Pavan AdiseshaiahSekhar P Reddy
Jan 17, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hue VuongSekhar P M Reddy
Feb 23, 2007·Molecular Cancer Research : MCR·Michalis V KaramouzisAthanasios G Papavassiliou
Feb 24, 2021·Journal of Cancer Research and Clinical Oncology·Tomonori SasahiraTadaaki Kirita

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.