Mechanism of rLcrV and rYopB mediated Immunosuppression in murine peritoneal macrophages

Molecular Immunology
Ajit SodhiUrmil Tuteja

Abstract

Yersinia pestis causative organism of bubonic and pneumonic plague uses type III secretion system for the translocation of effector molecules in to the target host cells. Type III secretion system of Yersinia pestis, secretes Low calcium response V (LcrV) or V antigen and many other Yersinia outer proteins (Yops). LcrV and YopB were initially known as translocator proteins, but recently have been shown to act as effector and regulatory proteins. Macrophages participate actively in immune response by secreting various effector molecules or by phagocytosis in clearing most of the pathogens. We investigated the effect of rLcrV and rYopB (10 microg/ml) on peritoneal macrophages in vitro. It is observed that rLcrV and rYopB inhibited LPS induced TNF-alpha and NO production in murine peritoneal macrophages. rLcrV and rYopB also inhibited expression of phospho-p38, -p42/44, -JNK MAPKs and transcription factors NFkappaB, c-fos and c-jun in LPS treated macrophages. The inhibition in the expression of these signaling molecules has been correlated to the inhibition of TNF-alpha and NO production in macrophages.

Citations

Mar 7, 2006·PLoS Pathogens·Masmudur M Rahman, Grant McFadden
Apr 28, 2009·Cellular Immunology·Andreas M LenzWilliam G Cheadle
Dec 21, 2007·Microbes and Infection·Bei LiRuifu Yang
May 28, 2005·Cellular Immunology·Dagmar Reithmeier-RostAndreas Sing
Jun 20, 2007·FEMS Microbiology Reviews·Anton ZavialovVladimir Zav'yalov
Mar 23, 2005·Molecular Immunology·Rajesh Kumar SharmaHarsh Vardhan Batra
Oct 13, 2006·Journal of Medical Microbiology·Andrey P Anisimov, Kingsley K Amoako
Mar 15, 2006·Journal of Medical Microbiology·Rekha KhushiramaniHarsh Vardhan Batra
Mar 25, 2009·International Journal of Immunopathology and Pharmacology·G Del PreteH S Mason

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.