Sep 7, 2017

Mechanism of salt-induced activity enhancement of a marine-derived laccase, Lac15

European Biophysics Journal : EBJ
Jie LiYazhong Xiao

Abstract

Laccase (benzenediol: oxygen oxidoreductases, EC1.10.3.2) is a multi-copper oxidase capable of oxidizing a variety of phenolic and other aromatic organic compounds. The catalytic power of laccase makes it an attractive candidate for potential applications in many areas of industry including biodegradation of organic pollutants and synthesis of novel drugs. Most laccases are vulnerable to high salt and have limited applications. However, some laccases are not only tolerant to but also activated by certain concentrations of salt and thus have great application potential. The mechanisms of salt-induced activity enhancement of laccases are unclear as yet. In this study, we used dynamic light scattering, size exclusion chromatography, analytical ultracentrifugation, intrinsic fluorescence emission, circular dichroism, ultraviolet-visible light absorption, and an enzymatic assay to investigate the potential correlation between the structure and activity of the marine-derived laccase, Lac15, whose activity is promoted by low concentrations of NaCl. The results showed that low concentrations of NaCl exert little influence on the protein structure, which was partially folded in the absence of the salt; moreover, the partially folded rat...Continue Reading

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Mentioned in this Paper

Analytical Ultracentrifugation
Study
Chloride Ion Level
L-2-hydroxyacid oxidase
Size
Exertion
Enzymes, antithrombotic
Organic Chemicals
Tertiary Protein Structure
Laccase B

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