Mechanism of selectivity in aquaporins and aquaglyceroporins.

Proceedings of the National Academy of Sciences of the United States of America
Jochen S Hub, Bert L de Groot

Abstract

Aquaporins and aquaglyceroporins form a family of pore proteins that facilitate the efficient and selective flux of small solutes across biological membranes. We studied the selectivity of aquaporin-1 (AQP1) and the bacterial glycerol facilitator, GlpF, for O(2), CO(2), NH(3), glycerol, urea, and water. Using molecular dynamics simulations, we calculated potentials of mean force for solute permeation along the aquaporin channels and compared them with the alternative pathway across the lipid bilayer. For small solutes permeating through AQP1, a remarkable anticorrelation between permeability and solute hydrophobicity was observed, whereas the opposite trend was observed for permeation through the membrane. This finding renders AQP1 a selective filter for small polar solutes, whereas GlpF was found to be highly permeable for small solutes and permeable for larger solutes. Surprisingly, not solute-channel but water-channel interactions were found to be the key determinant underlying the selectivity mechanism of aquaporins. Hence, a hydrophobic effect, together with steric restraints, determines the selectivity of aquaporins.

References

Aug 1, 1976·The Journal of General Physiology·A Finkelstein
Mar 1, 1990·Journal of Molecular Graphics·G Vriend
Jan 1, 1986·The Journal of Membrane Biology·A Walter, J Gutknecht
Mar 13, 1980·Biochimica Et Biophysica Acta·S A Simon, J Gutknecht
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·M J Borgnia, P Agre
May 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Morten Ø JensenKlaus Schulten
Oct 8, 2003·Journal of Molecular Biology·Bert L de GrootHelmut Grubmüller
Mar 30, 2004·Proteins·Boaz IlanGregory A Voth
Sep 2, 2004·Nature Reviews. Molecular Cell Biology·Landon S KingPeter Agre
Sep 29, 2004·Journal of Molecular Biology·Nilmadhab ChakrabartiRégis Pomès
Jul 1, 2005·Pflügers Archiv : European journal of physiology·Lars M HolmThomas Zeuthen
Sep 15, 2005·International Journal of Sports Medicine·S VogtA Schmid
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Eric BeitzThomas Zeuthen
May 10, 2006·Hypertension·Marcela HerreraJeffrey L Garvin
May 16, 2006·Biophysical Journal·Jochen S Hub, Bert L de Groot
Aug 19, 2007·Doklady. Biochemistry and Biophysics·I I IvanovA B Rubin
Apr 30, 1998·The Journal of Physical Chemistry. B·A D MacKerellM Karplus

❮ Previous
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Citations

Jun 10, 2009·European Biophysics Journal : EBJ·J Krüger, Wolfgang B Fischer
Jan 1, 2013·European Biophysics Journal : EBJ·Marcelo OzuOsvaldo Chara
Jul 3, 2008·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Ewan M CampbellAlan S Bowman
Aug 3, 2011·Pflügers Archiv : European journal of physiology·Marcela Herrera, Jeffrey L Garvin
Sep 17, 2013·Journal of Insect Physiology·Fernanda StaniscuaskiIan Orchard
May 17, 2008·Expert Reviews in Molecular Medicine·A S Verkman
Sep 30, 2009·Chemical Reviews·Ivet BaharIndira H Shrivastava
Mar 1, 2012·Journal of the American Chemical Society·Christian L WennbergJochen S Hub
Jan 15, 2013·Journal of the American Chemical Society·Günther H PetersPeter Westh
Dec 7, 2013·Nature Communications·Reginald McNultyMartin B Ulmschneider
Oct 15, 2008·Nature Clinical Practice. Endocrinology & Metabolism·Norikazu MaedaIichiro Shimomura
Jul 8, 2010·Physical Chemistry Chemical Physics : PCCP·Camilo Aponte-SantamaríaBert L de Groot
Mar 2, 2011·Physical Chemistry Chemical Physics : PCCP·L Y Chen
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·John C MathaiPeter Pohl
Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Prapasiri PongprayoonMark S P Sansom
Feb 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Romina OlivaMarialuisa Pellegrini-Calace
Jun 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Elena J LevinMing Zhou
Oct 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Hao DongMichael L Klein
Feb 9, 2012·The Journal of Biological Chemistry·Florian ZocherJohn C Mathai
May 18, 2012·The Journal of Biological Chemistry·Cecilia GeijerKarin Lindkvist-Petersson
Jun 17, 2010·Physiology·Yi WangEmad Tajkhorshid
Oct 25, 2012·PLoS Computational Biology·Eva ChovancovaJiri Damborsky
Mar 8, 2012·Molecular Medicine Reports·Yubao Cui, David A Bastien
Aug 21, 2012·International Journal of Biological Sciences·Yubao Cui, David A Bastien
Jan 14, 2014·Journal of Chemical Theory and Computation·Jochen S HubGerrit Groenhof
Feb 6, 2014·Journal of the American Chemical Society·Michiel van LunInger Andersson
Apr 18, 2014·The Journal of Physical Chemistry. B·Sara K HansenThomas Vosegaard
Mar 19, 2014·Acta histochemica·Antonia Debora SalesAna Paula Ribeiro Rodrigues
Sep 15, 2014·The Journal of Chemical Physics·Kun Huang, Angel E García
Oct 16, 2014·Applied Physics Letters·A Barati FarimaniEmad Tajkhorshid
Jul 1, 2009·Cellular and Molecular Life Sciences : CMLS·Uwe Ludewig, Marek Dynowski
Aug 21, 2013·The Journal of Physiology·Thomas ZeuthenNanna MacAulay
Jan 23, 2016·Anatomical Science International·Toshiyuki MatsuzakiKuniaki Takata
Oct 31, 2012·Journal of Structural Biology·Liao Y Chen
Jul 17, 2012·Plant Science : an International Journal of Experimental Plant Biology·Jaume FlexasCharles R Warren
Mar 20, 2012·Molecular Aspects of Medicine·Norikazu Maeda
Feb 22, 2012·Molecular Aspects of Medicine·Dan ZhangHe-Feng Huang

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