PMID: 20632935Jul 17, 2010Paper

Mechanism of steroidogenic electron transport: role of conserved Glu429 in destabilization of CYP11A1-adrenodoxin complex

Biochemistry. Biokhimii︠a︡
N V StrushkevichS A Usanov

Abstract

In the present work the role of conserved residue E429 of cytochrome P45011A1 has been studied. The charge neutralization of E429Q results in 3-fold decrease of K(d) as well as V(max) compared to the wild type hemoprotein indicating tighter binding and, as the result, the impaired dissociation of oxidized adrenodoxin from the complex. As cytochrome P45011A1-adrenodoxin complex formation is driven primarily by electrostatic interactions, the low activity of E429Q mutant is completely restored to that of wild type hemoprotein by increasing of ionic strength. The charge neutralization of the corresponding residue of rat cytochrome P45011B2 has the same effect: the activity is 10-fold decreased but it is restored by increasing of ionic strength without effect on the ratio of products formed. Thus, this is the first report on identification of residues involved in modulation of dissociation of redox partner from the complex with cytochrome P450s.

References

Dec 28, 1992·Biochimica Et Biophysica Acta·S Vijayakumar, J C Salerno
Jul 1, 1993·Biological & Pharmaceutical Bulletin·Y SagaraT Horiuchi
Jan 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·S VéronneauJ G LeHoux
Jan 6, 2000·Archives of Biochemistry and Biophysics·I A PikulevaY Kim
Jul 19, 2000·Proteins·A V GrinbergR Bernhardt
Oct 29, 2000·The Journal of Biological Chemistry·J J MüllerU Heinemann
Aug 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·James T WarrenLawrence I Gilbert
Oct 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Emily E ScottC David Stout
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·O GuryevR W Estabrook
Jun 8, 2004·The Journal of Biological Chemistry·Michael R WesterEric F Johnson
Jul 20, 2004·The Journal of Biological Chemistry·Jason K YanoEric F Johnson
May 31, 2008·Journal of Molecular Biology·Natallia StrushkevichHee-Won Park

❮ Previous
Next ❯

Citations

Jun 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Natallia StrushkevichHee-Won Park
May 8, 2019·Journal of Biomolecular Structure & Dynamics·Tayane Honorato Siqueira, Leandro Martínez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.