Mechanism of sulforaphane-induced cell cycle arrest and apoptosis in human colon cancer cells.

Nutrition and Cancer
Géraldine ParnaudL Gamet-Payrastre

Abstract

Sulforaphane (SFN) is a natural micronutrient found in cruciferous vegetables that has been shown to possess antitumoral properties in carcinogen-treated rats. In vitro, SFN regulates phase II enzymes, cell cycle, and apoptosis. In the present study, we investigated the relationship between SFN induction of apoptosis and cell cycle arrest in HT29 human colon carcinoma cells. In previously published data, a significant increase in the G2/M phase of the cell cycle has been observed in SFN-treated cells that was associated with increased cyclin B1 protein levels. In the present study, our results show that SFN induced p21 expression. Moreover, preincubation of HT29 cells with roscovitine, a specific cdc2 kinase inhibitor, blocked the G2/M phase accumulation of HT29 cells treated with SFN and abolished its apoptotic effect (22.2 +/- 4 of floating cells in SFN-treated cells vs. 6.55 +/- 2 in cells treated with both SFN and roscovitine). These results suggest that the cdc2 kinase could be a key target for SFN in the regulation of G2/M block and apoptosis. Moreover, in SFN-treated cells the retinoblastoma tumor suppressor protein (Rb) is highly phosphorylated. Inhibition of the cdc2 kinase by roscovitine did not change the phosphoryla...Continue Reading

References

Jan 1, 1990·Methods in Cell Biology·L Vindeløv, I J Christensen
Dec 1, 1973·Analytical Biochemistry·W Schaffner, C Weissmann
Nov 18, 1994·Cell·C J Sherr
Dec 1, 1995·Current Opinion in Cell Biology·G I EvanE Harrington
Apr 26, 1996·The Journal of Biological Chemistry·W Meikrantz, R Schlegel
Apr 18, 1998·Frontiers in Bioscience : a Journal and Virtual Library·Q P Dou, B An
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·S Y ArcherR A Hodin
May 20, 1999·Annual Review of Pharmacology and Toxicology·D G Johnson, C L Walker
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·S RaoK Keyomarsi
Oct 21, 1999·Experimental Cell Research·S L HsuW L Ho
Dec 10, 1999·The Journal of Biological Chemistry·P P HuX F Wang
Jun 19, 2001·The Journal of Biological Chemistry·E HeissC Gerhäuser
Sep 20, 2001·Current Medicinal Chemistry·M S LeviJ S Williamson
Dec 26, 2001·The Journal of Biological Chemistry·Yoshimasa NakamuraKoji Uchida
Jan 5, 2002·International Journal of Cancer. Journal International Du Cancer·Hong LinBen D Chen
Mar 6, 2002·The Journal of Biological Chemistry·Zhiyong HanJames H Wyche
May 2, 2002·Anti-cancer Drugs·Paul J Thornalley
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jed W FaheyAlain Lozniewski
Jan 18, 2003·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Todd R PraySusan D Demo

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Citations

Oct 12, 2013·The AAPS Journal·Yuanyuan LiTrygve O Tollefsbol
Oct 27, 2015·International Journal of Oncology·Dong Hwan KimNam Deuk Kim
Sep 13, 2015·Apoptosis : an International Journal on Programmed Cell Death·Hala Gali-MuhtasibNadine Darwiche
Nov 5, 2014·Antioxidants & Redox Signaling·Stephanie M TortorellaTom C Karagiannis
Dec 22, 2016·Critical Reviews in Food Science and Nutrition·Maria RussoSeyed Mohammad Nabavi
Jul 30, 2014·Journal of Bioscience and Bioengineering·Yuan-Kai ChungChia-Che Chang
Jan 26, 2017·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Ayako KawarazakiToshiyuki Sakai
Dec 19, 2015·Journal of the Science of Food and Agriculture·Nan WangLianqing Shen
May 22, 2009·The FEBS Journal·Yun-Xiang ZangBeom-Seok Park
Apr 11, 2017·Critical Reviews in Food Science and Nutrition·Jeong Hoon PanJae Kyeom Kim
Mar 24, 2004·Integrative Cancer Therapies·Anna-Sigrid Keck, John W Finley
Oct 5, 2018·The American Journal of Chinese Medicine·Hung-En ChenThomas I-Sheng Hwang
Dec 20, 2007·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Eun-Hye JoKyung-Sun Kang
Jul 11, 2006·Apoptosis : an International Journal on Programmed Cell Death·Juraj BodoJan Sedlak
Sep 5, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Jan-Ying YehPhilip D Whanger
Aug 29, 2007·Acta Pharmacologica Sinica·Yuesheng Zhang, Li Tang
Apr 21, 2019·Antioxidants·Melina MitsiogianniMihalis I Panayiotidis
Oct 22, 2008·American Journal of Respiratory and Critical Care Medicine·Hye-Youn ChoSteven R Kleeberger
Mar 23, 2011·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Hua YaoZhuo Zhang
Jul 28, 2005·The Journal of Nutrition·Maria TrakaRichard F Mithen
Sep 10, 2016·World Journal of Gastroenterology : WJG·Teng-Fei YinDong Wu
Sep 26, 2020·Frontiers in Genetics·Viviana J MancillaJamboor K Vishwanatha
Nov 18, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Prabhakaran Soundararajan, Jung Sun Kim
May 2, 2020·International Journal of Molecular Sciences·Jesús Santana-GálvezDaniel A Jacobo-Velázquez
Jun 30, 2019·Medicines·Tung-Chin ChiangAlan J Tackett
Dec 21, 2012·Oncology Reports·Yasushi SawaiShinji Fushiki

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