Mechanism of T cell proliferation in vivo: analysis of IL-2 receptor expression and activation of c-myc and c-myb oncogenes during lymphatic regeneration

Biochemical and Biophysical Research Communications
M SihvolaM Hurme

Abstract

The mechanism of T cell proliferation was studied using in vivo lymphatic regeneration as the model. Lymphatic regeneration was induced by injecting a sublethal dose (300 mg/kg) of cyclophosphamide (Cy) into mice. Majority of the regenerating splenic T cells were found to be in the cell cycle, nearly 30% being found in S/G2+M phases resembling the ratio obtained for mitogen activated T cells in vitro. Expression of interleukin-2 receptor (IL-2R) was defined by the monoclonal anti-IL-2R antibody, AMT-13. Only 1-3% of regenerating T cells were IL-2R positive (while about 30% of the in vitro activated T cells were IL-2R positive). Accordingly, these cells did not respond to IL-2 in vitro. However, when the freshly isolated regenerating T cells were cultured in the presence of Con A or PMA + ionophore A 23187, IL-2R was readily induced. The regenerating T cells were further analyzed for the expression of the cellular oncogenes c-myc and c-myb. These cells expressed about three times more c-myb mRNA than Con A-stimulated T cells and the levels were comparable to those seen in thymocytes. By contrast, the amount of c-myc mRNA was similar in the regenerating T cells and in Con A-activated T cells, but weak or barely detectable in sple...Continue Reading

References

Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·Z DarzynkiewiczM R Melamed
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T P Bender, W M Kuehl
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J C ReedR G Hoover
Feb 1, 1987·Proceedings of the Society for Experimental Biology and Medicine·M F SeldinA D Steinberg

❮ Previous
Next ❯

Citations

Nov 27, 1999·Mechanisms of Ageing and Development·N IshiyamaK Hirokawa
Jan 7, 2000·Experimental Cell Research·A EngelhardB Calabretta

❮ Previous
Next ❯

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.