Mechanism of the dynorphin-induced dualistic effect on free intracellular Ca2+ concentration in cultured rat spinal neurons

European Journal of Pharmacology
W H HuM F Jen

Abstract

In order to study the different mechanisms of dynorphin spinal analgesia and neurotoxicity at low and high doses, the effects of various concentrations of dynorphin A-(1-17) on the free intracellular Ca2+ concentration ([Ca2+]i) in the cultured rat spinal neurons were studied using single cell microspectrofluorimetry. While dynorphin A-(1-17) 0.1-100 microM had no significant effect on basal [Ca2+]i, dynorphin A-(1-17) 0.1 and 1 microM significantly decreased the high KCl-evoked peak [Ca2+]i by 94% and 83% respectively. Dynorphin A-(1-17) 10 and 100 microM did not affect the peak [Ca2+]i following K+ depolarization, but in all these neurons there was a sustained and irreversible rise in [Ca2+]i following high-K+ challenge. Pretreatment with the specific kappa-opioid receptor antagonist nor-binaltorphimine 10 microM, but not the competitive NMDA receptor antagonist, DL-2-amino-5-phosphonovalerate (APV) 10 microM, significantly blocked the inhibitory effect of dynorphin A-(1-17) 0.1 microM on peak [Ca2+]i. However, APV 10 microM and nor-binaltorphimine 10 microM significantly antagonized the sustained rise in [Ca2+]i induced by a high concentration of dynorphin A-(1-17) 10 microM. Furthermore, in the presence, and following the a...Continue Reading

References

Mar 1, 1992·Trends in Neurosciences·R Dubner, M A Ruda
Feb 1, 1990·Trends in Pharmacological Sciences·M J Millan
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R A Gross, R L Macdonald
Oct 24, 1989·European Journal of Pharmacology·D Massardier, P F Hunt
Mar 26, 1985·European Journal of Pharmacology·S Spampinato, S Candeletti
May 1, 1985·Regulatory Peptides·A I FadenB M Cox
May 1, 1988·Neuropeptides·I McFadzean
May 1, 1986·Annals of Neurology·S A Krumins, A I Faden
Jan 29, 1981·European Journal of Pharmacology·H J FriedmanH H Loh
Oct 18, 1982·Life Sciences·J S Han, C W Xie
Feb 1, 1995·The Journal of Physiology·L ChenL Y Huang
Dec 12, 1994·European Journal of Pharmacology·M Dumont, S Lemaire
Aug 22, 1994·European Journal of Pharmacology·J B Long, P Skolnick
Nov 1, 1994·Trends in Pharmacological Sciences·V K Shukla, S Lemaire

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Citations

Apr 2, 2003·Expert Opinion on Therapeutic Targets·Hai-Ying M Cheng, Josef M Penninger
Aug 31, 2014·Neuropharmacology·Ewelina RojewskaJoanna Mika
Sep 2, 2016·International Immunopharmacology·Xuxu XuJuan Feng
Feb 16, 1999·Journal of Neurotrauma·W H HuM F Jen

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