Mechanism of the Potential Therapeutic Candidate Bacillus subtilis BSXE-1601 Against Shrimp Pathogenic Vibrios and Multifunctional Metabolites Biosynthetic Capability of the Strain as Predicted by Genome Analysis

Frontiers in Microbiology
Dongdong WangXiangli Tian

Abstract

The global shrimp industry has suffered bacterial diseases caused mainly by Vibrio species. The typical vibriosis, acute hepatopancreatic necrosis disease (AHPND), has resulted in mass mortality and devastating economic losses. Thus, therapeutic strategies are highly needed to decrease the risk of vibriosis outbreaks. Herein, we initially identified that the growth of the causative agent of AHPND, Vibrio parahaemolyticus (VP AHPND ) and other vibrios in Pacific white shrimp (Litopenaeus vannamei) was inhibited by a Bacillus subtilis strain BSXE-1601. The natural products amicoumacins A, B, and C were purified from the cell-free supernatant from the strain BSXE-1601, but only amicoumacin A was demonstrated to be responsible for this anti-Vibrio activity. Our discovery provided the first evidence that amicoumacin A was highly active against shrimp pathogens, including the representative strain VP AHPND . Furthermore, we elucidated the amicoumacin A biosynthetic gene cluster by whole genome sequencing of the B. subtilis strain BSXE-1601. In addition to amicoumacin A, the strain BSXE-1601 genome harbored other genes encoding bacillibactin, fengycin, surfactin, bacilysin, and subtilosin A, all of which have previously reported a...Continue Reading

References

May 1, 1976·Journal of General Microbiology·M Kenig, E P Abraham
Feb 1, 1997·The Journal of Antibiotics·L M CañedoT García de Quesada
Mar 30, 2000·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·F R ChenK K Lee
Sep 13, 2003·BMC Bioinformatics·Roman L TatusovDarren A Natale
Jan 11, 2007·The Journal of Antimicrobial Chemotherapy·Charles E ShelburneMarilyn S Lantz
Jan 24, 2007·Bioinformatics·Arthur L DelcherSteven L Salzberg
Dec 21, 2007·Journal of Invertebrate Pathology·Siwaporn LongyantPaisarn Sithigorngul
May 10, 2008·Trends in Microbiology·Ashlee M EarlRoberto Kolter
Jul 28, 2009·Journal of Bacteriology·Tai HuangMichiko M Nakano
Nov 12, 2009·Diseases of Aquatic Organisms·Ken W HassonPatricia W Varner
Jan 31, 2012·International Journal of Systematic and Evolutionary Microbiology·Bruno Gomez-GilFabiano L Thompson
Apr 6, 2012·PloS One·Amrita LamaMichiko M Nakano
Mar 15, 2013·Molecular Biology and Evolution·Barry G Hall
Dec 21, 2013·Natural Product Reports·Daniela Reimer, Helge B Bode
Oct 14, 2014·Molecular Cell·Yury S PolikanovPetr V Sergiev
May 8, 2016·European Journal of Medicinal Chemistry·Aamer Saeed
Jun 15, 2016·Scientific Reports·Irina V ProkhorovaSergey E Dmitriev
Jun 28, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hyun Bong ParkJason M Crawford
Oct 30, 2016·Nucleic Acids Research·Baofeng JiaAndrew G McArthur

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Datasets Mentioned

BETA
CP028812
BSXE-1601

Methods Mentioned

BETA
PCR
electrophoresis
Assay
NMR
confocal

Software Mentioned

RNAmmer
Statistical Package for the Social Sciences ( SPSS )
AntiSMASH
SE
BLAST
tRNAscan
MEGA
SMRT Analysis
Glimmer
BLASTP

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