PMID: 42391Sep 15, 1979

Mechanism of the stimulation of serine and alanine transport into isolated rat liver cells by bicarbonate ions

The Biochemical Journal
J D McGivan


1. Bicarbonate ions stimulate the transport of serine and alanine into isolated hepatocytes. 2. The effect of bicarbonate is to increase the Vmax. of the transport process without changing the apparent Km. 3. The intracellular pH was estimated from the distribution of the weak base methylamine and the weak acid 5,5'-dimethyloxazolidine-2,4-dione (DMO) across the plasma membrane. 4. The addition of bicarbonate to a cell suspension caused the internal pH to become more acid. 5. The initial rate of serine, alanine and glycine transport was a linear function of the initial difference in pH across the membrane. 6. It is concluded that bicarbonate activates the transport of these amino acids primarily by increasing the pH difference across the plasma membrane. 7. It is suggested that the uptake of serine together with Na+ ions occurs in exchange for H+ ions, which are translocated outwards on the same carrier system. Some preliminary evidence consistent with this model is presented.


May 26, 2004·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·N CanoX M Leverve
Jul 1, 1983·Clinics in Endocrinology and Metabolism·R Park, A I Arieff
Jun 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·E PetzingerR K Kinne
Dec 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D J Stewart
Mar 8, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Brian MalesterWilliam A Coetzee
Dec 1, 1984·Archives internationales de physiologie et de biochimie·P FafournouxC Rémésy

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Carbonic Acid Ions
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