Mechanism of tracking and cleavage of adduct-damaged DNA substrates by the mammalian 5'- to 3'-exonuclease/endonuclease RAD2 homologue 1 or flap endonuclease 1.

The Journal of Biological Chemistry
C J BarnesRobert A Bambara

Abstract

The mammalian 5'- to 3'-exonuclease/endonuclease, called RAD2 homologue 1 or flap endonuclease 1, has a unique cleavage activity, dependent on specific substrate structure. On a primer-template, in which the primer has an unannealed 5'-tail, endonucleolytic cleavage near the annealing point releases the tail intact. Entering at the 5'-end, the nuclease tracks along the entire tail to the point of cleavage. Genetic analyses suggest that this nuclease removes DNA adducts in vivo (Sommers, C. H., Miller, E. J., Dujon, B., Prakash, S., and Prakash, L. (1995) J. Biol. Chem. 270, 4193-4196). Micrococcal nuclease footprinting shows that after tracking the nuclease protects a region of the tail 25 nucleotides long, adjacent to the cleavage site. Substrates with adducts at specific locations were used to assess the mechanism of RAD2 homologue 1 nuclease tracking and its ability to cleave modified DNA. Either a conventional cis-diamminedichloroplatinum (II) (CDDP) or a bulky CDDP derivative was placed within or beyond the region protected by the nuclease. The nuclease cleaved the tail of both substrates. In contrast, a CDDP adduct just adjacent to the expected cleavage point was inhibitory. A CDDP adduct at the very 5'-end of the tail wa...Continue Reading

References

Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G SiegalR A Bambara
May 2, 1990·Journal of the National Cancer Institute·C M SorensonA Eastman
Jan 17, 1991·Biochimica Et Biophysica Acta·R A Bambara, C B Jessee
Jan 1, 1990·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J L CanonM Symann
Sep 21, 1990·Cell·A MorrisonA Sugino
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Jan 1, 1989·Cancer Investigation·K J ScanlonH Miyachi
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·J J TurchiR A Bambara
Nov 10, 1995·The Journal of Biological Chemistry·M E BuddJ L Campbell
Aug 17, 1995·Nature·Y KimT A Steitz
Jan 1, 1995·Progress in Nucleic Acid Research and Molecular Biology·R A Bambara, L Huang
Mar 3, 1995·The Journal of Biological Chemistry·J J Harrington, M R Lieber
Dec 15, 1995·The Journal of Biological Chemistry·Y HabrakenS Prakash
Oct 1, 1995·Trends in Biochemical Sciences·D B Zamble, S J Lippard
Apr 19, 1996·The Journal of Biological Chemistry·B ShenM S Park
Oct 18, 1996·The Journal of Biological Chemistry·R S MuranteR A Bambara

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Feb 14, 2002·Nucleic Acids Research·Byung-in Lee BiDavid M Wilson
Aug 16, 2008·Molekuliarnaia biologiia·Zh K NazarkinaS N Khodyreva
Jun 11, 2004·Annual Review of Biochemistry·Yuan LiuRobert A Bambara
Mar 11, 1999·Current Opinion in Genetics & Development·R D Kolodner, G T Marsischky
Dec 12, 2012·Mutation Research·Anbarasi Kothandapani, Steve M Patrick
Sep 29, 2015·DNA Repair·Angela J Balliano, Jeffrey J Hayes
Dec 25, 2003·Critical Reviews in Biochemistry and Molecular Biology·Hui-I Kao, Robert A Bambara
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Oct 14, 2006·The Journal of Biological Chemistry·Jason A StewartRobert A Bambara
Sep 25, 1999·The Journal of Biological Chemistry·H WuS T Crooke
Apr 1, 2000·The Journal of Biological Chemistry·S TomR A Bambara
Apr 5, 2000·The Journal of Biological Chemistry·L A HenricksenR A Bambara
Feb 21, 1997·The Journal of Biological Chemistry·R A BambaraL A Henricksen
Oct 24, 1998·The Journal of Biological Chemistry·J A RumbaughR A Bambara
Mar 20, 2003·The Journal of Biological Chemistry·Marcel HohlOrlando D Schärer
Mar 24, 2004·The Journal of Biological Chemistry·Junzhuan QiuBinghui Shen
Jun 20, 1997·The Journal of Biological Chemistry·M WakasugiA Sancar
Oct 21, 1999·Biochemistry·C J BornarthR A Bambara

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