Abstract
The mammalian 5'- to 3'-exonuclease/endonuclease, called RAD2 homologue 1 or flap endonuclease 1, has a unique cleavage activity, dependent on specific substrate structure. On a primer-template, in which the primer has an unannealed 5'-tail, endonucleolytic cleavage near the annealing point releases the tail intact. Entering at the 5'-end, the nuclease tracks along the entire tail to the point of cleavage. Genetic analyses suggest that this nuclease removes DNA adducts in vivo (Sommers, C. H., Miller, E. J., Dujon, B., Prakash, S., and Prakash, L. (1995) J. Biol. Chem. 270, 4193-4196). Micrococcal nuclease footprinting shows that after tracking the nuclease protects a region of the tail 25 nucleotides long, adjacent to the cleavage site. Substrates with adducts at specific locations were used to assess the mechanism of RAD2 homologue 1 nuclease tracking and its ability to cleave modified DNA. Either a conventional cis-diamminedichloroplatinum (II) (CDDP) or a bulky CDDP derivative was placed within or beyond the region protected by the nuclease. The nuclease cleaved the tail of both substrates. In contrast, a CDDP adduct just adjacent to the expected cleavage point was inhibitory. A CDDP adduct at the very 5'-end of the tail wa...Continue Reading
References
Mar 9, 1979·Science·G L CohenS J Lippard
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G SiegalR A Bambara
May 2, 1990·Journal of the National Cancer Institute·C M SorensonA Eastman
Jan 17, 1991·Biochimica Et Biophysica Acta·R A Bambara, C B Jessee
Aug 13, 1991·Biochemistry·S F BellonS J Lippard
Jan 1, 1990·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J L CanonM Symann
Sep 21, 1990·Cell·A MorrisonA Sugino
Dec 1, 1990·Journal of Virology·T Nethanel, G Kaufmann
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Apr 1, 1990·Biophysical Chemistry·S F Bellon, S J Lippard
Jan 1, 1989·Cancer Investigation·K J ScanlonH Miyachi
Feb 21, 1989·Biochemistry·L Marrot, M Leng
Jun 26, 1989·Nucleic Acids Research· SibghatullahA Sancar
Jun 23, 1981·Biochemistry·H M UshayS J Lippard
Aug 1, 1982·Biochemical and Biophysical Research Communications·W M Scovell, V J Capponi
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·J J TurchiR A Bambara
Nov 10, 1995·The Journal of Biological Chemistry·M E BuddJ L Campbell
Jul 14, 1995·Science·R E JohnsonS Prakash
Aug 17, 1995·Nature·Y KimT A Steitz
Jan 1, 1995·Progress in Nucleic Acid Research and Molecular Biology·R A Bambara, L Huang
Mar 3, 1995·The Journal of Biological Chemistry·C H SommersL Prakash
Mar 3, 1995·The Journal of Biological Chemistry·J J Harrington, M R Lieber
Jun 1, 1994·Genes & Development·J J Harrington, M R Lieber
Oct 1, 1994·International Journal of Cancer. Journal International Du Cancer·K J MellishK R Harrap
Dec 1, 1994·International Journal of Cancer. Journal International Du Cancer·A E Oberli-SchrämmliT Cerny
Jul 1, 1994·Molecular and Cellular Biology·J M MurrayF Z Watts
Nov 25, 1993·Nature·Y HabrakenS Prakash
Jan 26, 1993·Biochemistry·L HuangR A Bambara
Dec 15, 1995·The Journal of Biological Chemistry·Y HabrakenS Prakash
Dec 22, 1995·The Journal of Biological Chemistry·R S MuranteR A Bambara
Oct 1, 1995·Trends in Biochemical Sciences·D B Zamble, S J Lippard
Apr 19, 1996·The Journal of Biological Chemistry·B ShenM S Park
Mar 22, 1996·The Journal of Biological Chemistry·J G MoggsR D Wood
Jul 4, 1996·Nature·T A CeskaD Suck
Jun 28, 1996·Cell·T C MueserC C Hyde
Oct 18, 1996·The Journal of Biological Chemistry·R S MuranteR A Bambara
Citations
Jun 18, 2002·Journal of Biomedical Science·Emmanuel A Faust, Henry Triller
Dec 21, 2006·Biochemistry·Aruna S JaiswalSatya Narayan
Aug 12, 1998·Nature Structural Biology·J R Sayers, P J Artymiuk
Jul 21, 2010·The Journal of Biological Chemistry·Aarthy C Vallur, Nancy Maizels
Aug 27, 2010·The Journal of Biological Chemistry·Jason W GloorRobert A Bambara
Nov 5, 1999·Human Molecular Genetics·A L GreeneM A Resnick
Jul 4, 2001·Nucleic Acids Research·S J GarforthJ R Sayers
Feb 14, 2002·Nucleic Acids Research·Byung-in Lee BiDavid M Wilson
Apr 4, 2006·Nucleic Acids Research·Ren LiuBinghui Shen
Feb 10, 2012·Nucleic Acids Research·Nikesh PatelJane A Grasby
Apr 30, 2008·Molecular and Cellular Biology·Zhigang GuoBinghui Shen
Aug 16, 2008·Molekuliarnaia biologiia·Zh K NazarkinaS N Khodyreva
Jun 11, 2004·Annual Review of Biochemistry·Yuan LiuRobert A Bambara
Feb 4, 2010·PloS One·Aarthy C Vallur, Nancy Maizels
Mar 11, 1999·Current Opinion in Genetics & Development·R D Kolodner, G T Marsischky
Dec 12, 2012·Mutation Research·Anbarasi Kothandapani, Steve M Patrick
Sep 29, 2015·DNA Repair·Angela J Balliano, Jeffrey J Hayes
Dec 25, 2003·Critical Reviews in Biochemistry and Molecular Biology·Hui-I Kao, Robert A Bambara
Jun 12, 2013·Cell Reports·Mohamed A SobhySamir M Hamdan
Apr 19, 2011·Cell·Jillian OransLorena S Beese
Oct 20, 1998·Cell·D J HosfieldJ A Tainer
Jul 31, 2002·The Journal of Biological Chemistry·Eriko MatsuiKazuaki Harata
Oct 14, 2006·The Journal of Biological Chemistry·Jason A StewartRobert A Bambara
Sep 25, 1999·The Journal of Biological Chemistry·H WuS T Crooke
Apr 1, 2000·The Journal of Biological Chemistry·S TomR A Bambara
Apr 5, 2000·The Journal of Biological Chemistry·L A HenricksenR A Bambara
Feb 21, 1997·The Journal of Biological Chemistry·R A BambaraL A Henricksen
Nov 22, 1996·The Journal of Biological Chemistry·M S DeMottS Zigman
May 18, 1999·The Journal of Biological Chemistry·J A RumbaughR A Bambara
Oct 24, 1998·The Journal of Biological Chemistry·J A RumbaughR A Bambara
Jun 22, 1999·The Journal of Biological Chemistry·E MatsuiI Matsui
Mar 20, 2003·The Journal of Biological Chemistry·Marcel HohlOrlando D Schärer
Mar 24, 2004·The Journal of Biological Chemistry·Junzhuan QiuBinghui Shen
Jun 20, 1997·The Journal of Biological Chemistry·M WakasugiA Sancar
Sep 16, 2004·Biochemistry·Anna PrzykorskaElzbieta Kuligowska
Oct 21, 1999·Biochemistry·C J BornarthR A Bambara
Apr 14, 1999·Biochemistry·J H YoonG P Pfeifer
Sep 25, 2002·Biochemistry·Devyani HaldarM R S Rao