Mechanism of translational coupling in the nifLA operon of Klebsiella pneumoniae

The EMBO Journal
F GovantesE Santero

Abstract

The nifLA operon of Klebsiella pneumoniae encodes the sensor-activator pair involved in the regulation of other nif genes. Balanced synthesis of both proteins, which is required for correct regulation, is achieved by coupling translation of nifA to that of nifL. The mechanism of translational coupling at the nifLA operon was analysed using a specialized ribosome system, and the effect of substituting the natural Shine-Dalgarno of nifL or nifA for specialized Shine-Dalgarno sequences was determined. Our results indicate that translational coupling occurs in this operon by a reinitiation mechanism. Additionally, reinitiation at the nifA can happen even in the absence of good Shine-Dalgarno recognition by the reinitiating ribosome, although its efficiency is lower. The effect of a putative translational enhancer sequence (downstream box) on translational coupling efficiency was also determined. Mutations that reduce the homology of the putative downstream box to the consensus had only a minor effect on nifA translation by wild-type ribosomes. However, they had a significant effect on nifA translation by specialized ribosomes, suggesting that recognition of the downstream box may compensate inefficient ribosomal interactions with t...Continue Reading

References

Apr 20, 1992·Journal of Molecular Biology·M Ivey-Hoyle, D A Steege
Mar 1, 1990·Trends in Genetics : TIG·J E McCarthy, C Gualerzi
Jun 20, 1990·Journal of Molecular Biology·M R Adhin, J van Duin
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A Hui, H A de Boer
Jul 1, 1989·Molecular & General Genetics : MGG·M R Adhin, J van Duin
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Jul 14, 1967·Journal of Molecular Biology·A Sarabhai, S Brenner
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Nov 1, 1994·Trends in Genetics : TIG·J E McCarthy, R Brimacombe
Mar 7, 1996·Molecular & General Genetics : MGG·F Govantes, E Santero

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Citations

Feb 13, 1999·Molecular Microbiology·S J StasinopoulosD H Bechhofer
Jun 5, 2008·Nucleic Acids Research·Lon M Chubiz, Christopher V Rao
Aug 23, 2006·Journal of Bacteriology·Patricia SkorskiSylvie Hermann-Le Denmat
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Brian Søgaard LaursenHans Uffe Sperling-Petersen
Aug 19, 2009·PLoS Biology·Linda LøvdokVictor Sourjik
Jun 20, 2006·Biochemical and Biophysical Research Communications·Sang-Joon LeeYounghoon Lee
Nov 5, 1999·Annual Review of Microbiology·C E BauerT H Bird
Sep 2, 2006·Microbiology·M Sofia Ciampi
Jul 13, 2000·The Journal of Biological Chemistry·R A NormanR E Drew

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