Mechanism of translational initiation in prokaryotes. Evidence for a direct effect of IF2 on the activity of the 30 S ribosomal subunit

FEBS Letters
M A CanonacoClaudio O Gualerzi

Abstract

Initiation factor IF2 from either Escherichia coli or Bacillus stearothermophilus was found to possess the previously undetected property of stimulating the template-dependent ribosomal binding of aminoacyl-tRNAs with free alpha-NH2 groups. IF1, which had no detectable activity alone, was found to stimulate the activity of E. coli IF2 and, to a lesser extent, that of B. stearothermophilus IF2. Since in the absence of ribosomes not even a weak interaction between the two IF2 molecules and the aminoacyl-tRNAs was detected, the present findings indicate that IF2 can act at the ribosomal level stimulating aminoacyl-tRNA binding without prior formation of a binary complex with the aminoacyl-tRNA. IF2 does not appear to open or strengthen a weak A-site binding, but rather to enhance aminoacyl-tRNA binding to a 30 S site equivalent to the P-site by slowing down the rate of aminoacyl-tRNA dissociation from ribosomes.

References

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Citations

May 1, 1995·Journal of Virological Methods·S R Kelsall, J K Kulski
May 3, 2000·The EMBO Journal·Jerneja TomsicClaudio O Gualerzi
Jun 23, 2012·Nucleic Acids Research·Attilio FabbrettiClaudio O Gualerzi
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Brian Søgaard LaursenHans Uffe Sperling-Petersen
Apr 27, 2012·PLoS Genetics·K Elizabeth MadisonHiroshi Nakai
May 26, 2006·The EMBO Journal·Ayman AntounMåns Ehrenberg
Aug 12, 2015·Cellular and Molecular Life Sciences : CMLS·Claudio O Gualerzi, Cynthia L Pon
Aug 29, 2006·Journal of Molecular Biology·Enrico CasertaClaudio O Gualerzi
Apr 21, 2012·Critical Reviews in Biochemistry and Molecular Biology·Pohl Milón, Marina V Rodnina
Jul 15, 2009·Molecular Cell·R Andrew MarshallJoseph D Puglisi
Mar 23, 2017·Molecular and Cellular Biology·Jalyce L E HellerHans-Joachim Wieden
Feb 6, 2010·Molecular Microbiology·Anna ZorzetDan I Andersson
Oct 8, 2014·The Journal of Biological Chemistry·Akesh SinhaUttam L RajBhandary
Mar 18, 2003·The Journal of Biological Chemistry·Alexei StortchevoiUttam L RajBhandary

Related Concepts

Geobacillus stearothermophilus
Alkalescens-Dispar Group
Peptide Chain Initiation, Translational
Peptide Initiation Factors
Gene Products, Protein
Ribosomes
RNA, Transfer, Amino Acyl
Eukaryotic Initiation Factor-2, gamma Subunit

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