Mechanism of trypsin-induced endothelium-dependent vasorelaxation in the porcine coronary artery

British Journal of Pharmacology
T NakayamaH Kanaide

Abstract

1. To investigate the mechanism underlying the trypsin-induced endothelium-dependent relaxation, cytosolic Ca(2+) concentration ([Ca(2+)](i)) and tension development of smooth muscle were simultaneously monitored in the porcine coronary artery, and [Ca(2+)](i) of in situ endothelial cells were monitored in the porcine aortic valvular strips, using fura-2 fluorometry. 2. During the contraction induced by 30 nM U46619, a thromboxane A(2) analogue, 100 nM trypsin induced a rapid transient significant decrease in both [Ca(2+)](i) (from 67.9+/-5.1 to 15.7+/-4.4%) and tension (from 97.5+/-9.2 to 16.8+/-3.5%) of smooth muscle only in the presence of endothelium (100% level was assigned to the level obtained with the 118 mM K(+)-induced contraction). [Ca(2+)](i) and the tension thus returned to the levels prior to the application of trypsin by 5 and 10 min, respectively. 3. The initial phase of this relaxation was partly inhibited by 100 microM N(omega)-nitro-L-arginine (L-NOARG), and was completely inhibited by L-NOARG plus 40 mM K(+) or L-NOARG plus 100 nM charybdotoxin and 100 nM apamin, while the late phase of the relaxation was inhibited by L-NOARG alone. 4. Trypsin induced a transient [Ca(2+)](i) elevation in the endothelial cell...Continue Reading

References

Oct 11, 1976·Biochimica Et Biophysica Acta·R S Temler, J P Felber
Oct 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D J AdamsC Van Breemen
Sep 15, 1989·Biochemical and Biophysical Research Communications·J Nishimura, C van Breemen
Jan 1, 1989·Methods in Cell Biology·F Di VirgilioS C Silverstein
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L J IgnarroG Chaudhuri
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·S NystedtJ Sundelin
Jun 16, 1997·FEBS Letters·N KoshikawaK Miyazaki
May 1, 1997·Journal of Vascular Research·I FlemingR Busse
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·W KongN W Bunnett
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·W F XuD C Foster
Jun 5, 1998·The American Journal of Physiology·O DéryN W Bunnett
Aug 11, 1998·European Journal of Pharmacology·O MizunoH Kanaide
Aug 26, 1998·Nature·M L KahnS R Coughlin
Jun 8, 1999·Current Opinion in Cardiology·R Brugada, R Roberts

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Citations

Apr 8, 2011·The Journal of Veterinary Medical Science·Daijiro KanekoKoichi Sato
Apr 3, 2010·Morphologie : Bulletin De L'Association Des Anatomistes·H RochaN Wafae
May 27, 2008·Morphologie : Bulletin De L'Association Des Anatomistes·T H M VieiraN Wafae
Jul 21, 2007·The American Journal of Pathology·Leon MoussaPeter G Tipping
Oct 21, 2003·Journal of Atherosclerosis and Thrombosis·Katsuya Hirano, Hideo Kanaide
Aug 26, 2004·American Journal of Physiology. Renal Physiology·Xuemei WangRodger Loutzenhiser
Nov 11, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Katsuya Hirano
Feb 3, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Tetsuhiro FujiyoshiHideo Kanaide

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