PMID: 9443939Jan 28, 1998Paper

Mechanism of YC-1-induced activation of soluble guanylyl cyclase

Molecular Pharmacology
Andreas Friebe, D Koesling

Abstract

The signaling molecule nitric oxide (NO) mediates many of its effects by the stimulation of soluble guanylyl cyclase (sGC). The activation process is initiated by high-affinity binding of NO to the enzyme's prosthetic heme group. Despite its poor sGC-activating properties, carbon monoxide (CO) has also been suggested as a physiological activator of sGC. Recently, we have shown that the substance YC-1, a benzyl indazole derivative, stimulates sGC by 10-fold (independently of NO) potentiates the stimulatory effect of NO, and turns CO into a potent activator of sGC. In the present study, we show that activation of sGC by protoporphyrin IX, a ligand-independent activator, was potentiated by YC-1, yet a shift of the concentration-response curve as seen with NO and CO was not observed. YC-1 slowed down the dissociation rates for NO and CO from the activated enzyme as monitored by cGMP accumulation after addition of the NO and CO scavenger oxyhemoglobin. A direct interaction of YC-1 with the heme group can be ruled out because YC-1 did not change the Soret absorption of basal or stimulated sGC and, in addition, still bound to the heme-depleted enzyme. Together, our results indicate that YC-1 increases the maximal catalytic rate and se...Continue Reading

References

Apr 15, 1991·Biochemical Pharmacology·J Utz, V Ullrich
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L J IgnarroG Chaudhuri
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L J IgnarroM S Wolin
Jan 15, 1993·Science·A VermaS H Snyder
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·V G KharitonovD Koesling
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·T MoritaS Kourembanas
Jan 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·R ZakharyS H Snyder
Feb 11, 1997·Biochemistry·A FriebeD Koesling

❮ Previous
Next ❯

Citations

Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·T C BellamyJ Garthwaite
Sep 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y C LeeF Murad
Apr 27, 2002·British Journal of Pharmacology·Tomas C Bellamy, John Garthwaite
Nov 20, 2002·Expert Opinion on Pharmacotherapy·J S KalsiS Minhas
Jun 28, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Nina Grosser, Henning Schröder
Jul 4, 2003·British Journal of Pharmacology·Barry J GibbJohn Garthwaite
Jun 8, 2004·Canadian Journal of Physiology and Pharmacology·Kinga JaronczykKanji Nakatsu
Oct 20, 2006·Journal of Pharmacological Sciences·Masaki NakaneJorge D Brioni
Jan 12, 2007·The EMBO Journal·Xiaolei MaFocco van den Akker
Mar 8, 2008·Pharmacological Reviews·Nader G Abraham, Attallah Kappas
Apr 21, 2009·Cell and Tissue Research·Grzegorz PodrygajloMichael Stern
Nov 11, 1999·British Journal of Pharmacology·M B HussainR J MacAllister
Apr 22, 2003·Journal of Cellular Physiology·William Durante
Oct 5, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Sönke BehrendsRalf Middendorff
Nov 1, 2001·Proceedings of the National Academy of Sciences of the United States of America·E MartinF Murad
Mar 22, 2005·The Journal of Pharmacology and Experimental Therapeutics·A Elizabeth LinderR Clinton Webb
Jul 26, 2003·Circulation Research·Andreas Friebe, Doris Koesling
Aug 1, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Mark R DuranskiDavid J Lefer
Jul 1, 2008·The European Journal of Neuroscience·John Garthwaite
Nov 14, 2006·The Journal of Biological Chemistry·Jonathan A WingerMichael A Marletta
Jun 3, 2008·The Journal of Biological Chemistry·Xiaohui HuWilliam R Montfort
May 2, 2008·Expert Opinion on Investigational Drugs·Andrew Feifer, Serge Carrier
May 25, 2011·Circulation·Johannes-Peter StaschOleg V Evgenov
Mar 12, 2011·Expert Opinion on Investigational Drugs·Ralph T SchermulyHossein Ardeschir Ghofrani
Sep 5, 2018·International Journal of Molecular Sciences·Ashfaq AhmadJoseph K Ritter
Mar 9, 2019·Biology of Reproduction·Damian D Guerra, K Joseph Hurt
Apr 30, 2002·The Journal of Biological Chemistry·Michael RusswurmDoris Koesling
Aug 28, 2009·American Journal of Physiology. Renal Physiology·Domingo TrigueroAngeles García-Pascual
Oct 23, 2003·The Journal of Biological Chemistry·Peter M SchmidtJohannes-Peter Stasch

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.