Mechanisms and consequences of neuronal stretch injury in vitro differ with the model of trauma

Journal of Neurotrauma
Donna M Geddes-KleinD F Meaney

Abstract

The deformation to the brain that occurs during traumatic brain injury (TBI) results in a complex strain distribution throughout the brain tissue. Recently, many in vitro models of neuronal injury have been developed to simplify the mechanics which occur during TBI. We hypothesized that the type of mechanical insult imparted onto neurons would significantly alter both the mechanism and severity of the neuronal response to injury. In this study, primary cortical neurons were cultured on an elastic substrate and subjected to graded levels (0%, 10%, 30%, 50%) of either uniaxial (cells stretched in one direction only) or biaxial (cells simultaneously stretched in two directions) stretch. We found that neurons stretched in either injury paradigm exhibited immediate increases in intracellular free calcium ([Ca2+]i), but the magnitude of the ([Ca2+]i) rise was nearly an order of magnitude higher in biaxially stretched neurons compared to uniaxially stretched neurons. Moreover, while the ([Ca2+]i) transient after uniaxial stretch was blocked with specific channel antagonists (APV, CNQX, nimodipine, TTX), a substantial ([Ca2+]i) transient persisted in biaxially stretched neurons. We theorized that the additional calcium influx after bia...Continue Reading

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Citations

Oct 14, 2011·Annals of Biomedical Engineering·Tapan P PatelDavid F Meaney
Sep 21, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Christopher MoraesCraig A Simmons
Apr 14, 2009·Médecine sciences : M/S·Marie-Michèle BeaulieuFrançois Berthod
Feb 23, 2012·Assay and Drug Development Technologies·Sarah SchmittVictor Sanjit Nirmalanandhan
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Dec 3, 2014·Archives of Medical Research·Serge C Thal, Winfried Neuhaus

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