Mechanisms and phenotypic consequences of acquisition of tigecycline resistance by Stenotrophomonas maltophilia

The Journal of Antimicrobial Chemotherapy
Paula BlancoJosé Luis Martinez

Abstract

To elucidate the potential mutation-driven mechanisms involved in the acquisition of tigecycline resistance by the opportunistic pathogen Stenotrophomonas maltophilia. The mutational trajectories and their effects on bacterial fitness, as well as cross-resistance and/or collateral susceptibility to other antibiotics, were also addressed. S. maltophilia populations were submitted to experimental evolution in the presence of increasing concentrations of tigecycline for 30 days. The genetic mechanisms involved in the acquisition of tigecycline resistance were determined by WGS. Resistance was evaluated by performing MIC assays. Fitness of the evolved populations and individual clones was assessed by measurement of the maximum growth rates. All the tigecycline-evolved populations attained high-level resistance to tigecycline following different mutational trajectories, yet with some common elements. Among the mechanisms involved in low susceptibility to tigecycline, mutations in the SmeDEF efflux pump negative regulator smeT, changes in proteins involved in the biogenesis of the ribosome and modifications in the LPS biosynthesis pathway seem to play a major role. Besides tigecycline resistance, the evolved populations presented cro...Continue Reading

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Citations

Feb 14, 2020·Expert Review of Anti-infective Therapy·Teresa Gil-GilPaula Blanco
Jan 15, 2021·International Journal of Molecular Sciences·María Blanca SánchezJosé Luis Martínez
May 28, 2021·Clinical Microbiology Reviews·Joanna S Brooke
Jun 19, 2021·Computational and Structural Biotechnology Journal·Teresa Gil-GilJosé Luis Martínez
Aug 7, 2021·Antibiotics·Viola Camilla ScoffoneSilvia Buroni
Aug 8, 2021·International Journal of Molecular Sciences·Fernando Sanz-GarcíaSara Hernando-Amado
Jun 27, 2020·European Journal of Microbiology & Immunology·Márió Gajdács, Edit Urbán

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