PMID: 9653450Jul 8, 1998Paper

Mechanisms determining sensitivity to cisplatin in three mutant Chinese hamster ovary cell lines

Mutation Research
E SegelovP R Harnett

Abstract

To gain insight into factors determining the response of tumours to cisplatin, we studied pathways involved in resistance to cisplatin: drug uptake, cytoplasmic detoxification and DNA repair, in three cisplatin-sensitive Chinese hamster ovary (CHO)2 mutant cell lines. The mutant lines, CHO-MMC6, CHO-MMC1, CHO-MMS2, displayed inherent sensitivity to cisplatin (2.2, 4.1 and 10.6-fold, respectively) compared to the CHO-K1 line from which they were derived. CHO-MMS2 was the only mutant to show sensitivity to UV and this was slight (< 2-fold). None of the mutants displayed increased sensitivity to X-irradiation. The CHO-MMS2 cell line appeared to have multiple mechanisms involved in its sensitivity to cisplatin, including increased drug accumulation, decreased levels of glutathione and a decreased capacity for DNA repair. The CHO-MMC1 mutant demonstrated reduced ability for DNA repair in a host cell reactivation assay, but no difference in drug accumulation or glutathione levels compared to the parent. The CHO-MMC6 cell line was not defective in any of the mechanisms studied. These three mutant cell lines demonstrate that similar mechanisms may account for inherent sensitivity or resistance to cisplatin, and suggest that multiple me...Continue Reading

References

Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A K GodwinM E Anderson
Mar 15, 1990·International Journal of Cancer. Journal International Du Cancer·R J SchilderT C Hamilton
Apr 5, 1989·Journal of the National Cancer Institute·G M LaiT C Hamilton
Apr 1, 1989·European Journal of Cancer & Clinical Oncology·P A AndrewsS B Howell
Apr 1, 1988·Trends in Genetics : TIG·I D Hickson, A L Harris
Apr 1, 1987·Carcinogenesis·C N Robson, I D Hickson
Dec 15, 1988·Biochemical Pharmacology·G M LaiT C Hamilton
Jun 15, 1987·International Journal of Cancer. Journal International Du Cancer·C R WolfJ F Smyth
May 1, 1980·Somatic Cell Genetics·L H ThompsonK Brookman
May 5, 1994·The New England Journal of Medicine·W C WoodC R Ferree
Jun 1, 1993·British Journal of Cancer·D P Gately, S B Howell
Oct 1, 1995·Trends in Biochemical Sciences·D B Zamble, S J Lippard
Mar 5, 1996·Biochemistry·J J TurchiK M Henkels

❮ Previous
Next ❯

Citations

Mar 8, 2000·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·L M AntunesM D Bianchi
Nov 5, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lyndee L ScurrAnna deFazio

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.