PMID: 9174649May 1, 1997Paper

Mechanisms involved in ATP-evoked Ca2+ oscillations in isolated human granulosa-luteal cells

Cell Calcium
P E SquiresA M Buchan

Abstract

Using single-cell microfluorimetry, we have shown that ATP evoked repetitive Ca2+ oscillations in intact Fura-2 loaded human granulosa-luteal cells (hG/LCs) in the absence of extracellular Ca2+. Sustained increases in [Ca2+]i required extracellular Ca2+ and ATP depleted stores were refreshed by brief (2 min) incubation with external Ca2+. Basal [Ca2+]i was unaffected by caffeine (1 mM), but 20 mM caffeine inhibited ATP-evoked Ca2+ release in the absence of external Ca2+. Thimerosal (10 microM) evoked repetitive Ca2+ spikes, under Ca(2+)-free conditions, which fused to form an elevated plateau when external Ca2+ was replaced. Thimerosal-induced changes in [Ca2+]i were reversibly inhibited by the thiol reducing agent dithiothreitol (1 mM). The periodicity and amplitude of the [Ca2+]i oscillations produced by thimerosal and ATP differ. ATP- or thimerosal-evoked changes in [Ca2+]i were unaffected by dantrolene sodium (10 microM). The Ca(2+)-ATPase inhibitor thapsigargin (1 microM) increased [Ca2+]i and attenuated subsequent ATP-evoked changes in [Ca2+]i. We conclude that ATP stimulates an oscillatory release of Ca2+ from InsP3-sensitive stores in hG/LCs.

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Citations

Nov 20, 2004·Cells, Tissues, Organs·Harshini Katugampola, Geoffrey Burnstock
Nov 23, 2013·Purinergic Signalling·Geoffrey Burnstock
Nov 26, 2013·Purinergic Signalling·Geoffrey Burnstock
May 12, 2015·Autonomic Neuroscience : Basic & Clinical·Ivana BjelobabaStanko S Stojilkovic
May 20, 2015·Autonomic Neuroscience : Basic & Clinical·George I Gorodeski
Apr 13, 2007·Physiological Reviews·Geoffrey Burnstock
Dec 21, 2019·Laboratory Investigation; a Journal of Technical Methods and Pathology·Woojin KangKenji Miyado
Apr 19, 2000·The Journal of Clinical Endocrinology and Metabolism·C J TaiP C Leung
Feb 7, 2001·The Journal of Clinical Endocrinology and Metabolism·C J TaiP C Leung
Jul 17, 1999·The American Journal of Physiology·J H Evans, M J Sanderson

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