Mechanisms involved in curcumin-induced human neutrophil apoptosis: Evidence that curcumin activates the endoplasmic reticulum stress-induced cell apoptosis pathway

SAGE Open Medicine
Francis Antoine, Denis Girard

Abstract

Curcumin was previously reported to accelerate neutrophil apoptosis, but the mechanism is unclear. Herein, we confirmed that curcumin induces human neutrophil apoptosis as assessed by cytology and by increase in the cell surface expression of annexin-V and CD16 shedding. Curcumin activated caspase-3 and the cleavage of the two cytoskeletal proteins lamin B1 and vimentin. In addition, curcumin activated protein kinase RNA-like endoplasmic reticulum kinase and eukaryotic initiation factor 2 alpha and reduced de novo protein synthesis and the protein expression of the two chaperone proteins, HSP70 and HSP90. We conclude that curcumin acts as an endoplasmic reticulum stressor in human neutrophils. The ability of curcumin to activate the endoplasmic reticulum stress-induced cell apoptotic pathway is part of its mode of action in primary cells like mature neutrophils.

References

Feb 13, 2001·FEBS Letters·C AkgulS W Edwards
Dec 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Martin PelletierDenis Girard
May 11, 2005·Biochemical and Biophysical Research Communications·Sudip SenNeeta Singh
Jan 7, 2006·Cell Death and Differentiation·M Boyce, J Yuan
Jan 3, 2007·Biochemical and Biophysical Research Communications·Hyun-Ock PaeHun-Taeg Chung
Jun 15, 2007·Nature Reviews. Molecular Cell Biology·David Ron, Peter Walter
Jun 16, 2007·Advances in Experimental Medicine and Biology·Bharat B AggarwalHaruyo Ichikawa
May 22, 2008·Apoptosis : an International Journal on Programmed Cell Death·Jason BakhshiRammohan V Rao
Dec 27, 2008·Trends in Pharmacological Sciences·Bharat B Aggarwal, Bokyung Sung
Nov 3, 2009·Biochemical and Biophysical Research Communications·François BinetDenis Girard
Dec 17, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·D M GonçalvesD Girard
Feb 9, 2010·International Immunopharmacology·François BinetDenis Girard
Jul 20, 2010·Immunological Reviews·Rodger DuffinAdriano G Rossi
Aug 14, 2010·Science·Darren Hutt, William E Balch
Oct 20, 2010·Current Drug Targets·Hongyu ZhouShile Huang
Jun 4, 2011·Cell Death and Differentiation·B Geering, H-U Simon
Sep 13, 2011·Cell·James E Rothman, Randy Schekman

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
fluorescence-activated cell sorting
electrophoresis
ubiquitination
protein folding

Software Mentioned

GraphPad Prism
GraphPad
FluorChem2

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis