PMID: 8604012Mar 1, 1996Paper

Mechanisms involved in eosinophil migration. Platelet-activating factor-induced chemotaxis and interleukin-5-induced chemokinesis are mediated by different signals

Journal of Leukocyte Biology
R C SchweizerL Koenderman

Abstract

Eosinophils play an important role in the pathogenesis of allergic diseases such as allergic asthma. Eosinophil migration in vitro can be divided into directed migration, or chemotaxis, and random migration, or chemokinesis. Here, we studied intracellular signals involved in eosinophil migration in vitro induced by platelet-activating factor (PAF) and interleukin-5 (IL-5), applying a Boyden chamber assay. Migration induced by PAF (10(-11)-10(-6) M) largely consisted of chemotaxis with some chemokinesis, whereas IL-5 (10(-12)-10(-8) M) induced chemokinesis only. Eosinophils were depleted from intracellular and extracellular Ca2+ to study the role of Ca2+ as a second messenger. Ca2+ depletion did not change PAF-induced chemotaxis, however, IL-5-induced chemokinesis was inhibited. Interestingly, PAF, but not IL-5, induced changes in [Ca2+]i. This rise originated mainly from internal stores. Inhibition of protein kinase A by H-89 and protein kinase C by GF 109203X had no effect on both forms of eosinophil migration. Addition of the protein kinase inhibitor staurosporine significantly inhibited IL-5-induced chemokinesis. Inhibition of tyrosine kinases by herbimycin A completely blocked IL-5-induced chemokinesis. PAF and IL-5-induced...Continue Reading

Citations

Apr 1, 1999·American Journal of Respiratory Cell and Molecular Biology·M J Tino, J R Wright
Jun 2, 2007·American Journal of Respiratory and Critical Care Medicine·Carmen M SwaisgoodEdward F Plow
Jan 22, 1998·Immunopharmacology and Immunotoxicology·M T VenturaA Tursi
Mar 6, 2007·Journal of Vascular Research·Manon M Oude NijhuisLaurien H Ulfman
Oct 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Laurien H UlfmanLeo Koenderman
Nov 1, 2006·Allergology International : Official Journal of the Japanese Society of Allergology·Takafumi YamaguchiMasahiko Kato
Aug 29, 2012·Journal of Leukocyte Biology·Vera M KampLeo Koenderman
Sep 26, 2013·PloS One·Vera M KampLeo Koenderman
Nov 22, 2000·Annals of Medicine·S Dunzendorfer, C J Wiedermann
Jul 14, 2001·Molecular Biology of the Cell·J AlblasL Koenderman
Mar 6, 2002·British Journal of Pharmacology·André KleinMauro M Teixeira
Aug 18, 2000·Immunology and Cell Biology·L Simson, P S Foster
Feb 22, 2005·International Archives of Allergy and Immunology·Choon-Sik ParkIl Yup Chung
Nov 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dominik HartlDirk Roos
Oct 9, 2001·The Journal of Allergy and Clinical Immunology·S DunzendorferC J Wiedermann
Jun 1, 1997·Immunology Letters·P J Coffer, L Koenderman

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