Abstract
Previous experiments have shown that acidosis enhances isoproterenol-induced phospholamban (PHL) phosphorylation (Mundiña-Weilenmann, C., Vittone, L., Cingolani, H. E., Orchard, C. H. (1996) Am. J. Physiol. 270, C107-C114). In the present experiments, performed in isolated Langendorff perfused rat hearts, phosphorylation site-specific antibodies to PHL combined with the quantitative measurement of 32P incorporation into PHL were used as experimental tools to gain further insight into the mechanism involved in this effect. At all isoproterenol concentrations tested (3-300 nM), phosphorylation of Thr17 of PHL was significantly higher at pHo 6.80 than at pHo 7.40, without significant changes in Ser16 phosphorylation. This increase in Thr17 phosphorylation was associated with an enhancement of the isoproterenol-induced relaxant effect. In the absence of isoproterenol, the increase in [Ca]o at pHo 6.80 (but not at pHo 7.40) evoked an increase in PHL phosphorylation that was exclusively due to an increase in Thr17 phosphorylation and that was also associated with a significant relaxant effect. This effect and the phosphorylation of Thr17 evoked by acidosis were both offset by the Ca2+/calmodulin-dependent protein kinase II inhibitor ...Continue Reading
References
Jan 25, 1977·Biochimica Et Biophysica Acta·M A Porzio, A M Pearson
Apr 1, 1992·Journal of Molecular and Cellular Cardiology·R NapolitanoA Mattiazzi
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Yoshimura, D M Cooper
Feb 1, 1992·The American Journal of Physiology·K W Spitzer, J H Bridge
Feb 1, 1992·The American Journal of Physiology·S M HarrisonC H Orchard
Mar 28, 1991·European Journal of Biochemistry·L K MacDougallP Cohen
Feb 1, 1990·The American Journal of Physiology·D C DevorM E Duffey
Apr 30, 1990·European Journal of Biochemistry·M J HubbardP Cohen
Jan 1, 1989·Annual Review of Biochemistry·P Cohen
Jul 23, 1982·Molecular and Cellular Biochemistry·M TadaF Ohmori
Aug 1, 1994·Cardiovascular Research·N ThanC H Orchard
Jul 7, 1993·Molecular and Cellular Biochemistry·L VittoneA Mattiazzi
Sep 1, 1995·Journal of Molecular and Cellular Cardiology·I EspinasseJ J Mercadier
Jan 1, 1996·The American Journal of Physiology·C Mundiña-WeilenmannC H Orchard
Dec 27, 1996·The Journal of Biological Chemistry·C Mundiña-WeilenmannA Mattiazzi
Citations
Jan 7, 2000·Biochemical and Biophysical Research Communications·E J MayerE McKenna
Jan 1, 2008·Apoptosis : an International Journal on Programmed Cell Death·Qiu-Jun YuFeng Gao
Jan 22, 2004·Journal of Molecular and Cellular Cardiology·J DeSantiagoD M Bers
Jul 19, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Steve R RoofMark T Ziolo
Jan 29, 2002·Journal of Molecular and Cellular Cardiology·Leticia VittoneAlicia Mattiazzi
Dec 13, 2000·Journal of Molecular and Cellular Cardiology·S BartelP Karczewski
Apr 15, 2009·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Naa-Adjeley AblorhJoel S Holger
Nov 6, 2004·The Journal of Physiology·Carlos A ValverdeAlicia Mattiazzi
Mar 1, 2012·Analytical Biochemistry·Naa-Adjeley AblorhDavid D Thomas
Oct 24, 2007·Journal of Molecular and Cellular Cardiology·Can M SagLars S Maier
Sep 11, 2007·Progress in Biophysics and Molecular Biology·Masanori KuzumotoSatoshi Matsuoka
Sep 15, 2000·Cardiology Clinics·K A Webster, N H Bishopric
Jan 16, 2007·Cardiovascular Research·Alicia MattiazziCecilia Mundiña-Weilenmann
Sep 18, 2002·Journal of Molecular and Cellular Cardiology·Lars S Maier, Donald M Bers
Sep 3, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·L S Maier
May 2, 2006·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·A MattiazziE G Kranias
Sep 16, 2003·The Journal of Biological Chemistry·Jeffrey J SaucermanAndrew D McCulloch
Feb 3, 2007·Circulation Research·Natalie BurkardOliver Ritter
Apr 5, 2003·Circulation Research·Lars S MaierDonald M Bers
May 29, 2014·Molecular Pharmacology·Robert K Amanfu, Jeffrey J Saucerman
Apr 6, 2019·American Journal of Respiratory Cell and Molecular Biology·Hannes HudallaHelen Christou
Sep 16, 2000·The Journal of Biological Chemistry·G ChuE G Kranias
Sep 28, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Carsten TschöpeRoland Vetter
May 24, 2003·American Journal of Physiology. Heart and Circulatory Physiology·M SaidA Mattiazzi
May 18, 1999·The American Journal of Physiology·M KuschelS Bartel
Mar 27, 2013·The Journal of Biological Chemistry·Kristiina RajamäkiKari K Eklund
Jun 14, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Noriyuki NomuraHideharu Hayashi
Jun 2, 2021·Cellular Signalling·Ruijie Liu