Mechanisms involved in the antiplatelet activity of tetramethylpyrazine in human platelets

Thrombosis Research
J R SheuM H Yen

Abstract

Tetramethylpyrazine is the active ingredient of a Chinese herbal medicine. In this study, tetramethylpyrazine was tested for its antiplatelet activities in human platelet suspensions. In human platelets, tetramethylpyrazine (0.5-1.5 mM) dose-dependently inhibited both platelet aggregation and ATP-release reaction induced by a variety of agonists (i.e., ADP, collagen, and U46619). Tetramethylpyrazine (0.5 mM) did not significantly change the fluorescence of platelet membranes labeled with diphenylhexatriene, even at the high concentration (1.5 mM). Furthermore, tetramethylpyrazine (0.5-1.5 mM) dose-dependently inhibited [3H]inositol monophosphate formation stimulated by collagen (5 microg/ml) in [3H]myoinositol loaded platelets. Tetramethylpyrazine (0.5-1.5 mM) also dose-dependently inhibited the intracellular free Ca2+ rise of Fura 2-AM loaded platelets stimulated by collagen (5 microg/ml). Moreover, tetramethylpyrazine (0.5-1.5 mM) inhibited thromboxane B2 formation stimulated by collagen. At a higher concentration (1.0 mM), tetramethylpyrazine has also been shown to influence the binding of FITC-triflavin to platelet glycoprotein IIb/IIIa complex. Triflavin, a specific glycoprotein IIb/IIIa complex antagonist purified from Tr...Continue Reading

References

Oct 18, 1976·Biochemical and Biophysical Research Communications·I F CharoT C Detwiler
Dec 15, 1992·Biochemical and Biophysical Research Communications·J R SheuT F Huang
Jul 15, 1991·The Biochemical Journal·T F HuangC M Teng
Apr 15, 1990·Thrombosis Research·S Y Liu, D M Sylvester
Sep 1, 1985·Journal of Cardiovascular Pharmacology·X Z Dai, R J Bache
Jun 1, 1987·British Journal of Pharmacology·C B Neylon, R J Summers
Jan 1, 1989·Stroke; a Journal of Cerebral Circulation·W K HoC M Lee
Jan 1, 1985·Advances in Experimental Medicine and Biology·D E MacIntyreA McNicol
Feb 1, 1972·British Journal of Haematology·J F MustardM A Packham
Dec 18, 1981·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R H MichellJ A Creba
Oct 1, 1981·Biochemical Society Transactions·C J KirkR H Michell
Aug 1, 1963·The Journal of Physiology·G V BORN, M J CROSS

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Citations

Sep 10, 2002·Journal of Biomedical Science·Chi-Feng LiuSong-Chow Lin
May 10, 2001·International Journal of Hematology·J R SheuM H Yen
Oct 1, 2003·Clinical and Experimental Pharmacology & Physiology·Kar-Lok WongJuei-Tang Cheng
Jun 26, 2015·Neural Regeneration Research·Hai-Jun GaoHai-Yan Huang
Oct 11, 2015·Chinese Journal of Integrative Medicine·Ying ZhangDa-Zhuo Shi
Mar 26, 2014·Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan·Xiaojia NiXinfeng Guo
Jun 16, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Cen ChenZhi-Ning Xia
Apr 8, 2016·Expert Opinion on Drug Metabolism & Toxicology·Thanasekaran JayakumarJoen-Rong Sheu
Jul 28, 2016·Phytochemistry·Duo-Duo DingYi Kong
Jun 29, 2017·Journal of Biochemical and Molecular Toxicology·Yi-Chien FangChung-Ren Jan
Aug 19, 2007·Journal of Cardiovascular Pharmacology·Genshan MaZhong Chen
May 26, 2018·Singapore Medical Journal·Jia Wei LimTang Ching Lau
Nov 6, 2018·International Journal of Molecular Medicine·Li LiKeji Chen

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