Mechanisms involved in the blood-testis barrier increased permeability induced by EMP

Toxicology
XiaoWu WangGuoZhen Guo

Abstract

The blood-testis barrier (BTB) plays an important role in male reproductive system. Lots of environmental stimulations can increase the permeability of BTB and then result in antisperm antibody (AsAb) generation, which is a key step in male immune infertility. Here we reported the results of male mice exposed to electromagnetic pulse (EMP) by measuring the expression of tight-junction-associated proteins (ZO-1 and Occludin), vimentin microfilaments, and transforming growth factor-beta (TGF-beta3) as well as AsAb level in serum. Male BALB/c mice were sham exposed or exposed to EMP at two different intensities (200kV/m and 400kV/m) for 200 pulses. The testes were collected at different time points after EMP exposure. Immunofluorescence histocytochemistry, western blotting, laser confocal microscopy and RT-PCR were used in this study. Compared with sham group, the expression of ZO-1 and TGF-beta3 significantly decreased accompanied with unevenly stained vimentin microfilaments and increased serum AsAb levels in EMP-exposed mice. These results suggest a potential BTB injury and immune infertility in male mice exposed to a certain intensity of EMP.

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Citations

Feb 6, 2014·Biological Trace Element Research·Kangchu LiGuozhen Guo
Jun 13, 2012·Journal of Natural Science, Biology, and Medicine·S M J MortazaviA Rajaie-Far
Jun 19, 2014·Electromagnetic Biology and Medicine·Congsheng LiTongning Wu
Mar 26, 2013·Archives of Medical Research·Da-peng JiangGuo-zhen Guo
Jun 27, 2012·Annals of the New York Academy of Sciences·Lila G Glotfelty, Gail A Hecht
Sep 21, 2011·International Journal of Radiation Biology·Yong Bin ChenG Z Guo
Mar 1, 2019·International Journal of Molecular Sciences·Ning QuKou Sakabe
Dec 13, 2019·BMC Complementary and Alternative Medicine·Kumpei TakahashiMasahiro Itoh
Dec 15, 2020·Journal of Human Reproductive Sciences·Chidiebere Emmanuel Okechukwu

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