Mechanism(S) Involved in the Colon-Specific Expression of the Thiamine Pyrophosphate (Tpp) Transporter.

PloS One
Svetlana M NabokinaHamid M Said

Abstract

Microbiota of the large intestine synthesizes considerable amount of vitamin B1 (thiamine) in the form of thiamine pyrophosphate (TPP). We have recently demonstrated the existence of an efficient and specific carrier-mediated uptake process for TPP in human colonocytes, identified the TPP transporter (TPPT) involved (product of the SLC44A4 gene), and shown that expression of TPPT along the gastrointestinal (GI) tract is restricted to the colon. Our aim in this study was to determine the molecular basis of the colon-specific expression of TPPT focusing on a possible epigenetic mechanism. Our results showed that the CpG island predicted in the SLC44A4 promoter is non-methylated in the human colonic epithelial NCM460 cells, but is hyper-methylated in the human duodenal epithelial HuTu80 cells (as well as in the human retinal pigment epithelial ARPE19 cells). In the mouse (where TPPT expression in the GI tract is also restricted to the colon), the CpG island predicted in the Slc44a4 promoter is non-methylated in both the jejunum and colon, thus arguing against possible contribution of DNA methylation in the colon-specific expression of TPPT. A role for histone modifications in the tissue-specific pattern of Slc44a4 expression, howe...Continue Reading

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Citations

May 17, 2017·Molecular Pharmaceutics·Henrike BruckmuellerStefan Oswald
Dec 10, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Veedamali S SubramanianHamid M Said
Nov 20, 2018·Natural Product Reports·Lauren J Rajakovich, Emily P Balskus
Sep 22, 2017·American Journal of Physiology. Cell Physiology·Kasin Yadunandam AnandamHamid M Said
Jul 21, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kalidas RamamoorthyHamid M Said
Sep 16, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ram LakhanHamid M Said
Mar 25, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Subrata SabuiHamid M Said

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Methods Mentioned

BETA
Assay
transfection
PCR
immunoprecipitation
electrophoresis
acetylation

Software Mentioned

- CDS
Basic Local Alignment Search Tool ( BLAST )
DIANA microT
miRanda
miRDB
QUMA
INCELL
Methprimer
BLASTN
TargetScanS

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