Mechanisms involved in the nitric oxide independent inhibitory neurotransmission to the pig urinary bladder neck

Neurourology and Urodynamics
Ana Martínez-SáenzMedardo Hernández

Abstract

The current study investigates the mechanisms involved in nitric oxide (NO)-independent, nonadrenergic, noncholinergic (NANC) inhibitory neurotransmission to the pig urinary bladder neck. Urothelium-denuded strips were mounted in organ baths containing physiological saline solution (PSS) at 37°C for isometric force recordings. The relaxations to electrical field stimulation (EFS) were carried out on strips treated with guanethidine, atropine and N(G) -nitro-L-arginine, to block noradrenergic neurotransmission, muscarinic receptors and NO synthase, respectively, and precontracted with phenylephrine. EFS (1-16 Hz) produced frequency-dependent relaxations which were abolished by the blockade of neuronal voltage-activated Na(+) channels. Nonselective and selective inhibition of COX and COX-1, respectively, and blockade of Na(+) -K(+) ATPase reduced the EFS-induced relaxations. However, blockade of COX-2, soluble guanylyl cyclase, large-, intermediate- and small-conductance Ca(2+) -activated K(+) channels, ATP-dependent K(+) channels, voltage-gated K(+) channels, cAMPc-dependent protein kinase (PKA) and cGMP-dependent protein kinase (PKG) failed to modify the nerve-mediated relaxations. The NO-independent inhibitory neurotransmissio...Continue Reading

References

Jul 31, 1990·Biochemical and Biophysical Research Communications·L J IgnarroJ Rajfer
Dec 1, 1993·The American Journal of Physiology·A D Bonev, M T Nelson
Jan 23, 1999·The Journal of Urology·S F EnglishE J McGuire
Jul 18, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·G M HerreraM T Nelson
Oct 5, 2001·Trends in Pharmacological Sciences·R Schubert, M T Nelson
Apr 8, 2003·The Journal of Physiology·Kevin S Thorneloe, Mark T Nelson
Dec 17, 2004·Pharmacological Reviews·Karl-Erik Andersson, Alan J Wein
Apr 23, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Gerald M HerreraMark T Nelson
Feb 9, 2006·British Journal of Pharmacology·William C de Groat
Feb 3, 2007·Neurourology and Urodynamics·Medardo HernándezLuis M Orensanz
Mar 25, 2009·British Journal of Pharmacology·Paz RecioMedardo Hernández
Jul 1, 2009·British Journal of Pharmacology·Medardo HernándezGeoffrey Burnstock
Dec 22, 2009·Neurourology and Urodynamics·Karl-Erik Andersson

❮ Previous
Next ❯

Citations

Dec 4, 2013·European Journal of Pharmacology·Vítor S FernandesMedardo Hernández
Jun 21, 2011·The Journal of Urology·Ana Martínez-SáenzMedardo Hernández
Oct 16, 2012·The Journal of Urology·Vítor S FernandesMedardo Hernández
Sep 29, 2011·Neurourology and Urodynamics·José Luis ArteagaMedardo Hernández
Mar 31, 2012·Neurourology and Urodynamics·José Luis ArteagaMedardo Hernández
Jan 31, 2012·Steroids·Vítor S FernandesMedardo Hernández

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.