Mechanisms involved in the stimulation of prostacyclin synthesis by human lymphocytes in human umbilical vein endothelial cells

British Journal of Pharmacology
Faten Merhi-SoussiAnnie-France Prigent

Abstract

1 Endothelial cells play an important role in the modulation of vascular tone because of their ability to produce vasoactive substances such as prostacyclin (PGI(2)). Cell-cell contact between human umbilical vein endothelial cells (HUVEC) and peripheral blood lymphocytes has been shown to stimulate endothelial PGI(2) synthesis by increasing free arachidonic acid availability through endothelial cytosolic phospholipase A2 (cPLA(2)) activation. In this study, we sought to determine whether phospholipase C (PLC) and D (PLD) activation also contributes, besides cPLA(2), to the lymphocyte-induced PGI(2) synthesis in HUVEC, and to delineate further the potential mechanisms of cPLA(2) activation triggered by the interaction of HUVEC with lymphocytes. 2 Pretreatment of endothelial cells with the PI-PLC inhibitor U-73122 before the coincubation with lymphocytes markedly inhibited the PGI(2) output whereas the diacylglycerol (DAG) lipase inhibitor RHC 80267 and ethanol had no effect. These results suggest that PLC may be involved through inositol trisphosphate generation and calcium mobilization, and that neither DAG nor phosphatidic acid (PtdOH) was used as sources of arachidonic acid. 3 The stimulated PGI(2) synthesis was protein kina...Continue Reading

References

Oct 21, 1992·Molecular and Cellular Biochemistry·V W van Hinsbergh
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D T DudleyA R Saltiel
Apr 12, 1996·Molecular and Cellular Biochemistry·C H YuV Panagia
Oct 1, 1995·Journal of Lipid Mediators and Cell Signalling·J D ClarkL L Lin
Apr 1, 1997·Current Opinion in Cell Biology·D K Morrison, R E Cutler
Jul 4, 1997·The Journal of Biological Chemistry·C C Leslie
Nov 5, 1997·Analytical Biochemistry·A J MorrisJ Engebrecht
Apr 18, 1998·The Journal of Biological Chemistry·J T WongP C Choy
Jul 11, 1998·The Journal of Biological Chemistry·M F FavataJ M Trzaskos
Jun 4, 1999·Progress in Biophysics and Molecular Biology·M H Cobb
Jul 30, 1999·Biochimica Et Biophysica Acta·L W DanielS Ghosh
Dec 2, 1999·Experimental Cell Research·J EnglishM H Cobb
Nov 18, 2000·Biochimica Et Biophysica Acta·T Hirabayashi, T Shimizu

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Citations

May 5, 2004·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·I Marrero, A Déniz
May 8, 2010·European Journal of Pharmacology·Robert NowakJolanta Tarasiuk

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