Mechanisms of agonist-induced constriction in isolated human mesenteric arteries

Vascular Pharmacology
Joanne HallRichard D Jones

Abstract

We determined the calcium signalling pathways involved in the mechanisms of contraction of the vasoconstrictive agonists KCl, U46619 and PDBu in isolated human mesenteric arteries. The influence of gender, vessel diameter and age of the patients was also investigated. Human mesenteric arteries (n = 76) were loaded in a wire myograph and maintained at a tension equivalent to the in vivo pressure of 100 mm Hg, bubbled with 95%O2/5%CO2 to maintain pH 7.4 in physiological saline solution (PSS). Cumulative concentration-response curves were obtained to KCl (100 microM-100 mM), U46619 (1 nM-1 microM) or PDBu (1 nM-1 microM), before or after a 30 min incubation with either the voltage-gated calcium channel (VGCC) blocker nifedipine (10 microM), the store-operated calcium channel (SOCC) blocker SK&F96365 (50 microM) or in calcium-free PSS (-Ca2+ PSS). The KCl response was abolished in -Ca2+ PSS and with nifedipine. The U46619 response was partially blocked in -Ca2+ PSS and with nifedipine and predominantly blocked by SK&F96365. Incubation in -Ca2+ PSS had no effect on the response to PDBu. Arteries from male patients responded significantly higher to KCl than arteries from female patients. This study demonstrates that KCl induces mesen...Continue Reading

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Citations

Apr 9, 2008·Pediatric Research·Beatrix S TraaAnne M Comi
Aug 14, 2012·BMC Complementary and Alternative Medicine·Arif-ullah KhanDharmani Devi Murugan
Mar 12, 2009·Vascular Pharmacology·Joanne HallRichard D Jones
May 8, 2007·Vascular Pharmacology·Daniela Z DimitrovaDessislava B Duridanova
Sep 22, 2010·Phytotherapy Research : PTR·Arif-ullah Khan, Anwarul Hasan Gilani
Aug 26, 2010·Basic & Clinical Pharmacology & Toxicology·Min Tae KimSeon Jeong Kim
Oct 30, 2016·Basic & Clinical Pharmacology & Toxicology·Martin GrannUlf Simonsen
Jan 31, 2014·Biology of Reproduction·Christina E HaywardMark Wareing
Mar 26, 2019·Clinical and Experimental Pharmacology & Physiology·Hong YanChun-Yu Deng
Sep 21, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Connie J MarkKathleen M Eyster
Jul 30, 2020·Prostaglandins & Other Lipid Mediators·Gulsev OzenGokce Topal
Apr 14, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Nadeem IrshadMuhammad Shahid Iqbal

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