Mechanisms of androgen induction of sebocyte differentiation

Dermatology : International Journal for Clinical and Investigative Dermatology
R L RosenfieldN Ciletti

Abstract

It has been difficult to induce the expected sebocyte differentiation in vitro with dihydrotestosterone (DHT). We reasoned that our culture system lacks differentiating factors, and peroxisome proliferator-activated receptors (PPARs) were the prime candidates. We tested PPAR activators informative about diverse PPAR subtypes, with and without DHT (10(-6) M): BRL-49653 (10(-6) M, PPAR-gamma), WY-14643 (10(-6) M, PPAR-alpha), and linoleic acid (LIN, 10(-4) M, PPAR-delta). Treatments were added in serum-free medium to cultures of rat preputial sebocytes. Control, DHT, BRL and BRL + DHT treatments caused 11, 25, 66 and 80%, respectively, of preputial cell colonies to differentiate into lipid-forming colonies (LFCs) (p < 0.001). WY induced 20% and LIN over 95% LFC formation. PPAR-gamma mRNA was identified in preputial sebocytes by the RNase protection assay. These data suggest that differentiation of sebocytes is transduced by PPARs and have implications for the development of new treatments for acne.

Citations

Jan 29, 2010·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·C C Zouboulis
May 19, 2010·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·E Makrantonaki, C C Zouboulis
Jun 1, 2010·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·C C Zouboulis
Jan 3, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Theodosios AlestasChristos C Zouboulis
Aug 12, 2008·Archives of Dermatological Research·Christos C ZouboulisTheodosios Alestas
Mar 9, 2011·Pflügers Archiv : European journal of physiology·Balázs I TóthTamás Bíró
Nov 16, 2002·Prostaglandins & Other Lipid Mediators·Gerhard FürstenbergerPeter Krieg
Feb 15, 2002·Journal of the European Academy of Dermatology and Venereology : JEADV·H Gollnick
Jan 25, 2013·BMC Dermatology·Adrian J McNairnGéraldine Guasch
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Christos C ZouboulisStefan R Bornstein
Aug 3, 2011·Journal of Nutrition and Metabolism·Dianne DeplewskiRobert L Rosenfield
Apr 20, 2002·In Vitro Cellular & Developmental Biology. Animal·Robert L RosenfieldNancy Ciletti
Apr 25, 2009·Current Opinion in Endocrinology, Diabetes, and Obesity·Evgenia Makrantonaki, Christos C Zouboulis
Sep 11, 2012·Seminars in Cell & Developmental Biology·Catherin Niemann, Valerie Horsley
Jun 30, 2015·The Journal of Investigative Dermatology·Kai KretzschmarFiona M Watt
Aug 20, 2010·Experimental Gerontology·Evgenia MakrantonakiChristos C Zouboulis
Nov 26, 2009·The Medical Clinics of North America·Margarita S LolisAlan R Shalita
Nov 22, 2005·The Journal of Investigative Dermatology·Christos C Zouboulis
May 6, 2006·The Journal of Investigative Dermatology·Nishit R TrivediDiane M Thiboutot
Mar 17, 2007·The Journal of Investigative Dermatology·Wesley J HarrisonMichael P Philpott
Jan 19, 2006·The Journal of Investigative Dermatology·Qiwei ZhangRaymond L Konger
Jun 12, 2004·The Journal of Investigative Dermatology·Diane Thiboutot
Jul 10, 2001·The Journal of Investigative Dermatology·S B ShappellA R Brash
Feb 16, 1999·The Journal of Investigative Dermatology·R L RosenfieldN Ciletti
Feb 19, 2008·Clinics in Dermatology·F William Danby
Oct 28, 2004·Experimental Dermatology·Christos C Zouboulis, Klaus Degitz
Aug 23, 2003·The British Journal of Dermatology·S Kuenzli, J-H Saurat
Nov 22, 2005·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Christos C Zouboulis
Dec 21, 2006·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Volker NiemeierUwe Gieler
Jul 16, 2008·Journal of the European Academy of Dermatology and Venereology : JEADV·Y PangH D Chen
Mar 18, 2011·Experimental Dermatology·Qiang JuChristos C Zouboulis
Feb 16, 2010·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Bodo C Melnik
May 15, 2010·International Journal of Dermatology·Gang FuTian Yang
Nov 24, 2004·Clinics in Dermatology·Christos C Zouboulis
Nov 3, 2004·Journal of Dermatological Science·Shigeki InuiSatoshi Itami
Jan 1, 2015·Nature Reviews. Disease Primers·Sara Moradi TuchayiChristos C Zouboulis
Apr 20, 2001·The Journal of Veterinary Medical Science·E IwataY Mori
Jul 19, 2011·Clinical Biochemistry·Megha Kataria AroraVandana Saini
Apr 27, 2011·Dermato-endocrinology·Evgenia MakrantonakiChristos Zouboulis
Mar 13, 2010·Dermato-endocrinology·Christos C Zouboulis

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