Mechanisms of bacillus Calmette-Guerin mediated natural killer cell activation

The Journal of Urology
Henrik SuttmannSven Brandau

Abstract

Natural killer (NK) cells are of crucial importance for bacillus Calmette-Guerin (BCG) mediated antitumor effects. We defined the mechanisms of BCG mediated NK cell activation in vitro. A standard Cr release assay was used to measure the cytotoxicity of BCG activated NK cells. Using the MACS system (Miltenyi Biotec, Bergisch-Gladbach, Germany) we depleted various immune cell subpopulations from BCG stimulated peripheral blood mononuclear cells to phenotype activated NK cells. During the stimulation process anticytokine antibodies and recombinant cytokines were added to define their role in NK cell activation. For costimulation studies peripheral blood mononuclear cells were separated into lymphocytes and monocytes by counterflow-centrifugation (elutriation). Inhibitory NK cell receptor expression on activated NK cells was measured by flow cytometry by antiCD3, antiCD56 and anti-inhibitory NK cell receptor triple staining. The accessory function of monocytes was indispensable for BCG mediated NK cell activation. However, the stimulatory potential of monocytes did not require direct cell-cell contact to NK cells or major histocompatibility complex dependent antigen presentation to T cells. Monocyte derived interleukin (IL)-12 and...Continue Reading

References

Sep 1, 1993·The Journal of Urology·T L RatliffW J Catalona
Feb 1, 1996·Current Opinion in Immunology·A MeliánM B Brenner
May 1, 1996·Immunology Today·M López-BotetJ Strominger
May 21, 1999·Lancet·A B AlexandroffK James
Aug 23, 2000·Immunology Today·L MorettaA Moretta
Mar 13, 2001·Annual Review of Immunology·J L Flynn, J Chan
May 8, 2001·International Journal of Cancer. Journal International Du Cancer·S BrandauA Böhle
Jul 21, 2001·European Urology·S Brandau, A Böhle
Nov 8, 2001·Trends in Immunology·M A CooperM A Caligiuri
Mar 7, 2002·International Reviews of Immunology·K S Campbell, M Colonna
Aug 13, 2003·The Journal of Urology·Andreas Böhle, Sven Brandau
Sep 17, 2003·Tissue Antigens·T CabreraF Garrido

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Citations

Jun 26, 2013·Immunology and Cell Biology·Daniel W FowlerMark D Bodman-Smith
May 6, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·M Firoz MianAli A Ashkar
Jul 20, 2010·Immunotherapy·Anton B AlexandroffAndrew M Jackson
May 11, 2011·Immunotherapy·Pierre L TriozziErnest Borden
Mar 4, 2014·Seminars in Immunology·Mariella Della ChiesaAlessandro Moretta
Oct 9, 2012·Seminars in Oncology·Vinod H NargundM N Kabir
Feb 22, 2011·Journal of Theoretical Biology·Svetlana Bunimovich-MendrazitskyJoel Chaskalovic
Oct 16, 2007·The Journal of Urology·Thomas S Griffith
Jan 3, 2008·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Marie-Claude RousseauYves St-Pierre
Feb 5, 2014·Nature Reviews. Urology·Gil Redelman-SidiBernard H Bochner
Jul 12, 2018·Nature Reviews. Urology·Caroline Pettenati, Molly A Ingersoll
Mar 13, 2021·Frontiers in Immunology·Gloria EstesoMar Valés-Gómez
Aug 15, 2019·European Urology Oncology·Concepción F GuillamónAlfredo Minguela

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