Mechanisms of capsid assembly around a polymer.

Biophysical Journal
Aleksandr Kivenson, Michael F Hagan

Abstract

Capsids of many viruses assemble around nucleic acids or other polymers. Understanding how the properties of the packaged polymer affect the assembly process could promote biomedical efforts to prevent viral assembly or nanomaterials applications that exploit assembly. To this end, we simulate on a lattice the dynamical assembly of closed, hollow shells composed of several hundred to 1000 subunits, around a flexible polymer. We find that assembly is most efficient at an optimum polymer length that scales with the surface area of the capsid; polymers that are significantly longer than optimal often lead to partial-capsids with unpackaged polymer "tails" or a competition between multiple partial-capsids attached to a single polymer. These predictions can be tested with bulk experiments in which capsid proteins assemble around homopolymeric RNA or synthetic polyelectrolytes. We also find that the polymer can increase the net rate of subunit accretion to a growing capsid both by stabilizing the addition of new subunits and by enhancing the incoming flux of subunits; the effects of these processes may be distinguishable with experiments that monitor the assembly of individual capsids.

References

Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·A ZlotnickP T Wingfield
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·M A KrolP Ahlquist
Mar 30, 2002·Science·George M Whitesides, Bartosz Grzybowski
Jul 19, 2002·Biophysical Journal·Dan Endres, Adam Zlotnick
Jun 11, 2004·Biophysical Journal·Willem K Kegel, Paul van der Schoot Pv
Feb 9, 2005·Physical Review Letters·Joseph Rudnick, Robijn Bruinsma
Mar 17, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Alexander McPherson
Aug 3, 2005·Nature Reviews. Microbiology·Victoria D'Souza, Michael F Summers
Aug 11, 2005·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Paul van der Schoot, Robijn Bruinsma
Oct 6, 2005·Nature Reviews. Microbiology·Padmaja NatarajanJohn E Johnson
Feb 14, 2006·Virology·Paul GraysonRob Phillips
Mar 28, 2006·Biophysical Journal·Michael F Hagan, David Chandler
May 23, 2006·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Daniel George AngelescuPer Linse
Sep 14, 2006·Nano Letters·Suraj K DixitBogdan Dragnea
Oct 10, 2006·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Stephen D Hicks, C L Henley
Nov 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Vladimir A Belyi, M Muthukumar
Dec 14, 2006·Organic & Biomolecular Chemistry·Friso D SikkemaRoeland J M Nolte
Jan 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jingchuan SunBogdan Dragnea
Feb 15, 2007·Nano Letters·Hung D NguyenCharles L Brooks
Apr 17, 2007·Journal of Molecular Biology·Peter G StockleyAlison E Ashcroft
Aug 7, 2007·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Tao Hu, B I Shklovskii
Sep 4, 2007·The Journal of Chemical Physics·Alex W WilberPauline Wong
Oct 13, 2007·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Robert L JackDavid Chandler
Oct 24, 2007·The Journal of Chemical Physics·Stephen Whitelam, Phillip L Geissler
Nov 13, 2007·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Nicholas A Licata, Alexei V Tkachenko
Dec 1, 2007·Journal of Molecular Biology·Katerina ToropovaNeil A Ranson
Apr 4, 2008·The European Physical Journal. E, Soft Matter·D G AngelescuR F Bruinsma
Jun 4, 2008·Physical Review Letters·Se Il Lee, Toan T Nguyen
Jul 23, 2008·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Michael F Hagan
Oct 11, 2008·Proceedings of the National Academy of Sciences of the United States of America·Aron M YoffeAvinoam Ben-Shaul
Oct 22, 2008·Biophysical Journal·Roya Zandi, Paul van der Schoot
Oct 28, 2008·Nano Letters·Oren M Elrad, Michael F Hagan
Nov 13, 2008·Physical Review Letters·D C Rapaport
Dec 31, 2008·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Antonio Siber, Rudolf Podgornik
Feb 10, 2009·Journal of the American Chemical Society·Hung D NguyenCharles L Brooks
Mar 26, 2009·The Journal of Chemical Physics·Michael F Hagan
Apr 23, 2009·Biophysical Journal·Tao JiangJianzhong Wu
Oct 29, 2009·Proceedings of the National Academy of Sciences of the United States of America·Nolwenn JouvenetPaul D Bieniasz
Nov 10, 2009·The Journal of Chemical Physics·Alex W WilberArd A Louis
Feb 11, 2010·Physical Chemistry Chemical Physics : PCCP·Stephen C HarveyMustafa Burak Boz

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Citations

Feb 15, 2012·Journal of the American Chemical Society·Stanislav KlerUri Raviv
Oct 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Christina L TingZhen-Gang Wang
Dec 15, 2010·Physical Biology·Oren M Elrad, Michael F Hagan
Nov 19, 2013·PLoS Computational Biology·Guido PollesCristian Micheletti
Aug 3, 2012·The Journal of Chemical Physics·Zhehui Jin, Jianzhong Wu
Apr 26, 2013·The Journal of Chemical Physics·Ran Zhang, Per Linse
Apr 10, 2012·The Journal of Chemical Physics·J P Mahalik, M Muthukumar
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Eric C DykemanReidun Twarock
Jun 26, 2013·ELife·Jason D PerlmutterMichael F Hagan
Nov 27, 2015·Journal of Molecular Biology·Óttar RolfssonPeter G Stockley
Feb 5, 2015·Biophysical Journal·Teresa Ruiz-Herrero, Michael F Hagan
Feb 14, 2016·Biochimica Et Biophysica Acta·Tyler Reddy, Mark S P Sansom
Jan 27, 2012·Current Opinion in Structural Biology·Marissa G Saunders, Gregory A Voth
Dec 24, 2014·Annual Review of Physical Chemistry·Jason D Perlmutter, Michael F Hagan
Apr 9, 2013·Biophysical Journal·Adam ZlotnickJoseph Che-Yen Wang
Aug 17, 2011·Biophysical Journal·Karim M ElSawyReidun Twarock
May 12, 2016·ELife·Jason D PerlmutterMichael F Hagan
Apr 10, 2017·The Journal of Chemical Physics·Daniel G Angelescu
Sep 19, 2015·Chemical Society Reviews·Lin ZhangNatalie K Connors
Mar 9, 2018·Chemistry : a European Journal·Stan J MaassenJeroen J L M Cornelissen
Apr 6, 2019·Annual Review of Biophysics·Reidun Twarock, Peter G Stockley
Jul 28, 2011·Angewandte Chemie·Charlotte Uetrecht, Albert J R Heck
Nov 26, 2010·The Journal of Biological Chemistry·Yannick J BombleMichael F Crowley
Mar 25, 2011·Physical Chemistry Chemical Physics : PCCP·A G Cherstvy
Mar 19, 2013·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Eric C DykemanReidun Twarock
Aug 2, 2019·Nano Letters·Margherita MarchettiWouter H Roos

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