Abstract
Twenty-nine Pseudomonas aeruginosa isolates, which are resistant to carbapenems but susceptible to ceftazidime or/and cefepime, were recovered from our hospital from July 2011 to October 2011. The results of Western blotting showed that the OprD was reduced or lost. None of the 29 clinical isolates produced carbapenemases, extended-spectrum β-lactamases, or Ambler class C β-lactamases enzymes by the modified 3-dimensional test. The sequencing of oprD for these isolates showed that there are multiple point mutations, large fragment substitutions, deletions, and insertions. It showed that the expression of oprD decreased while mexA and mexX increased by real-time reverse transcriptase-PCR. These results suggested that the loss of OprD and overexpression of mexXY-OprM and mexAB-OprM are associated with carbapenem resistance in cephalosporin-susceptible Pseudomonas aeruginosa.
References
Jan 22, 2003·Environmental Microbiology·Jean-Paul PirnayMartin Zizi
Jun 24, 2004·FEMS Microbiology Letters·Daniel J WolterPhilip D Lister
Mar 30, 2006·Antimicrobial Agents and Chemotherapy·Didier HocquetPatrick Plésiat
Jun 17, 2009·Journal of Medical Microbiology·Tanya Strateva, Daniel Yordanov
Sep 10, 2009·Antimicrobial Agents and Chemotherapy·José-Manuel Rodríguez-MartínezPatrice Nordmann
May 24, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A B Campo EsquisabelL Martínez-Martínez
Citations
Feb 13, 2016·Jundishapur Journal of Microbiology·Huseyin Agah TerziIhsan Hakki Ciftci
Oct 7, 2015·PloS One·Jilu ShenYaping Fang
May 21, 2016·Future Microbiology·Theodoros KarampatakisEmmanuel Roilides
Jun 28, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Mohammad Taghi AkhiVajihe Sheikhalizadeh
Nov 15, 2017·Antimicrobial Agents and Chemotherapy·Yu Mi WiRobin Patel
Apr 5, 2017·Antimicrobial Agents and Chemotherapy·Yu Mi WiKwan Soo Ko
Nov 12, 2019·Acta Microbiologica Et Immunologica Hungarica·Younes KhaliliMohammad Yousef Memar
Feb 23, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Manju SureshManish Kumar Panickassery Ramakrishnan
Jan 6, 2016·The Journal of Microbiology·Chi Hyun KimJe Chul Lee
Nov 9, 2018·Scientific Reports·Gertrudis HornaJoaquim Ruiz
Apr 5, 2020·Antibiotics·Márió Gajdács
Jul 28, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sameer S KadriUNKNOWN National Institutes of Health Antimicrobial Resistance Outcomes Research Initiative (NIH–ARORI)
Nov 16, 2018·Indian Journal of Medical Microbiology·Agila Kumari PragasamKeith S Kaye
Jan 8, 2021·Antibiotics·Payam BehzadiMárió Gajdács