Mechanisms of carbapenem resistance in cephalosporin-susceptible Pseudomonas aeruginosa in China

Diagnostic Microbiology and Infectious Disease
Zhang-Rui ZengHai-Feng Shao

Abstract

Twenty-nine Pseudomonas aeruginosa isolates, which are resistant to carbapenems but susceptible to ceftazidime or/and cefepime, were recovered from our hospital from July 2011 to October 2011. The results of Western blotting showed that the OprD was reduced or lost. None of the 29 clinical isolates produced carbapenemases, extended-spectrum β-lactamases, or Ambler class C β-lactamases enzymes by the modified 3-dimensional test. The sequencing of oprD for these isolates showed that there are multiple point mutations, large fragment substitutions, deletions, and insertions. It showed that the expression of oprD decreased while mexA and mexX increased by real-time reverse transcriptase-PCR. These results suggested that the loss of OprD and overexpression of mexXY-OprM and mexAB-OprM are associated with carbapenem resistance in cephalosporin-susceptible Pseudomonas aeruginosa.

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Citations

Feb 13, 2016·Jundishapur Journal of Microbiology·Huseyin Agah TerziIhsan Hakki Ciftci
May 21, 2016·Future Microbiology·Theodoros KarampatakisEmmanuel Roilides
Jun 28, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Mohammad Taghi AkhiVajihe Sheikhalizadeh
Apr 5, 2017·Antimicrobial Agents and Chemotherapy·Yu Mi WiKwan Soo Ko
Nov 12, 2019·Acta Microbiologica Et Immunologica Hungarica·Younes KhaliliMohammad Yousef Memar
Feb 23, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Manju SureshManish Kumar Panickassery Ramakrishnan
Nov 9, 2018·Scientific Reports·Gertrudis HornaJoaquim Ruiz
Jul 28, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sameer S KadriUNKNOWN National Institutes of Health Antimicrobial Resistance Outcomes Research Initiative (NIH–ARORI)
Nov 16, 2018·Indian Journal of Medical Microbiology·Agila Kumari PragasamKeith S Kaye

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