Mechanisms of cell damage due to mechanical impact: an in vitro investigation.

Scientific Reports
Wonmo KangMarc Raphael

Abstract

The dynamic response of cells when subjected to mechanical impact has become increasingly relevant for accurate assessment of potential blunt injuries and elucidating underlying injury mechanisms. When exposed to mechanical impact, a biological system such as the human skin, brain, or liver is rapidly accelerated, which could result in blunt injuries. For this reason, an acceleration of greater than > 150 g is the most commonly used criteria for head injury. To understand the main mechanism(s) of blunt injury under such extreme dynamic threats, we have developed an innovative experimental method that applies a well-characterized and -controlled mechanical impact to live cells cultured in a custom-built in vitro setup compatible with live cell microscopy. Our studies using fibroblast cells as a model indicate that input acceleration ([Formula: see text]) alone, even when it is much greater than the typical injury criteria, e.g., [Formula: see text] g, does not result in cell damage. On the contrary, we have observed a material-dependent critical pressure value above which a sudden decrease in cell population and cell membrane damage have been observed. We have unambiguously shown that (1) this critical pressure is associated wit...Continue Reading

References

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Dec 2, 2017·Acta Biomaterialia·Wonmo KangAmit Bagchi
Oct 28, 2018·Scientific Reports·Wonmo Kang, Marc Raphael
Jan 15, 2019·ACS Applied Materials & Interfaces·Bo ChenMichael Cho

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Citations

Jul 3, 2021·Life·Chunghwan KimWonmo Kang

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Methods Mentioned

BETA
imaging techniques

Software Mentioned

Incucyte
SignalExpress

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