Mechanisms of cell injury in rat mesentery and cremaster muscle

The American Journal of Physiology
A G HarrisG W Schmid-Schönbein

Abstract

The events responsible for cell injury after a tissue stimulation are only incompletely understood. The purpose of this study was to examine mechanisms of cell injury in two tissues, rat mesentery and cremaster muscle, after tissue stimulation with N-formylmethionyl-leucyl-phenylalanine (FMLP) and platelet-activating factor (PAF). The response was studied in the same animal in random order using normal and leukopenic rats. The tissues were exteriorized after pentobarbital anesthesia. Five to six vascularized areas were chosen in each tissue, and cell injury and hydroperoxide production were assessed visually by continuous superfusion with 1 microM propidium iodide and 5 microM dichlorofluorescin diacetate (DCFH), respectively. FMLP (1 x 10(-8) M) and then PAF (1 x 10(-8) M) were added to the superfusate, and measurements were made at several time points. The second tissue was then examined using the same protocol. In the cremaster, there was little hydroperoxide production, and the tissue injury was eliminated after leukopenia. Leukopenia had no effect on tissue injury in the mesentery. Although hydroperoxide production was observed, there was no correlation between it and the tissue injury. The level of preactivation showed no...Continue Reading

Citations

Mar 10, 2000·American Journal of Physiology. Heart and Circulatory Physiology·S Bertuglia, A Colantuoni
Oct 23, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Longkun ZhuPingnian He
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Min ZengPingnian He
Sep 23, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hannah J ZhangKevin C Kregel
Oct 23, 2002·Journal of Applied Physiology·Dawn R S SteinerJohn G Wood
Nov 23, 2012·Journal of Applied Physiology·Nadi T WickramasekeraDavid R Harder
Mar 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Shinya TakaseGeert W Schmid-Schönbein
Dec 7, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Nathalie BaudryEric Vicaut

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