PMID: 6966822Jan 1, 1980Paper

Mechanisms of cell-mediated cytotoxicity in mice rejecting xenogeneic human lymphoblastoid cells

Scandinavian Journal of Immunology
C CarnaudR Van der Gaag

Abstract

The in vivo immunization of mice with human lymphoblastoid cell line LNH13 generates direct cell-mediated cytotoxicity by spleen cells. The lytic activity appears as early as day 3 after the intraperitoneal inoculation of 7.5 x 10(6) cells and persists at least until day 11. The killer cells do not adhere to plastic and are not retained on nylon wool columns or on Degalan beads coated with mouse Ig plus rabbit-anti-mouse Ig. The effector cells are partly inhibited by treatment with anti-Thy-1.2 serum plus complement, but this inhibition appears to be non-specific since anti-serum alone or normal serum plus complement have the same effects. Heat-aggregated IgG strongly inhibits cytotoxicity, indicating that the effector cells are Fc-positive and that such receptors are implicated in lysis. Altogether, these features strongly argue for an ADCC phenomenon. The involvement of antibodies is demonstrated by the fact that eluates (56 degrees C, 30 min) from immune cells alone induce lysis in the presence of normal spleen cells as effectors. The lytic activity of these eluates can be removed by specific adsorption on protein A coupled to Sepharose beads and on the human lymphoid target cells. Positive complementation between immune and...Continue Reading

References

Feb 1, 1977·European Journal of Immunology·C CarnaudJ F Bach
Aug 1, 1976·The Journal of Experimental Medicine·K F Lindahl, F H Bach
Jan 1, 1977·Scandinavian Journal of Immunology·A K KimuraL C Andersson
Jun 1, 1978·The Journal of Experimental Medicine·M Jondal, S Targan
Jan 1, 1979·Immunological Reviews·R Kiessling, H Wigzell
Jan 1, 1979·Immunological Reviews·R B HerbermanP Puccetti
Feb 1, 1976·Cellular Immunology·I A Ramshaw, C R Parish
Jan 1, 1974·Advances in Immunology·J C Cerottini, K T Brunner
Feb 1, 1972·European Journal of Immunology·M L Lohmann-MatthesH Fischer
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg
Dec 1, 1971·The Journal of Experimental Medicine·P GolsteinH Wigzell

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenocortical Carcinoma

Adrenocortical carcinoma (ACC) is a rare malignancy of the adrenal cortex, associated with a generally dismal prognosis owing to its aggressive behavior. Here are the latest discoveries pertaining to this disease.

Antibody-Dependent Cell Cytotoxicity

Antibody-dependent cellular toxicity refers to the lysis of a target cell by a non-sensitized effector cell of the immune system as a result of antibodies binding to the target cell membrane and engaging the Fc receptors on the immune effector cells. Find the latest research on antibody-dependent cellular toxicity here.

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.