PMID: 51710Aug 11, 1975Paper

Mechanisms of chromosome banding. VII. Interaction of methylene blue with DNA and chromatin

Chromosoma
D E Comings, E Avelino

Abstract

The binding of methylene blue to DNA and chromatin treated in various ways was examined by equilibrium dialysis. The maximum r value (moles of bound dye/mole of nucleotide) was 1.0 for DNA, 0.6 for unfixed chromatin, and 0.83 for chromatin fixed in methanol-acetic acid. When fixed chromatin was treated with saline-citrate at 60 degrees C for 3 hours, as used for G-banding chromosomes, the r value decreased from 0.83 to 0.55. When unfixed chromatin was treated as for R-banding the r values also dropped. Equilibrium dialysis indicated there was no disproportionate increase of dye binding as the concentration of DNA increased. -- These results, and others, suggest that some of the Giemsa negative regions of G- and R-banded chromosomes are due to the denaturation of non-histone proteins so that they more effectively cover the DNA and prevent side binding of the thiazin dyes.

References

Jul 7, 1971·Nature: New Biology·A T SumnerR A Buckland
Mar 15, 1973·Experimental Cell Research·D E ComingsH E Wyandt
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S A Latt
May 1, 1974·Experimental Cell Research·D E Comings, E Avelino
Jan 1, 1973·Cytogenetics and Cell Genetics·S Pathak, F E Arrighi
Jan 1, 1974·Cytogenetics and Cell Genetics·D M Kurnit
Jan 1, 1974·Chromosoma·J R Korenberg, E F Freedlender
Jan 1, 1973·Annual Review of Biochemistry·W J RutterP W Morris
Aug 5, 1974·Biochemical and Biophysical Research Communications·L AugenlichtR Baserga
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S Y Lin, A D Riggs
Mar 25, 1973·Journal of Molecular Biology·R F Itzhaki, H K Cooper
Sep 1, 1973·Journal of Medical Genetics·M A Ferguson-Smith, B M Page
Mar 30, 1974·Experimental Cell Research·B Dutrillaux, M Covic
Apr 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J D ReganR D Ley
Jan 27, 1971·Nature: New Biology·R J Clark, G Felsenfeld
Oct 9, 1970·Biochemical and Biophysical Research Communications·R F Itzhaki
Sep 26, 1967·Biochimica Et Biophysica Acta·A Miura, Y Ohba

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Citations

Jan 1, 1983·Human Genetics·F A BignoneG Gimelli
May 1, 1978·Stain Technology·K Noda, K J Kasha
Apr 12, 2003·Archives of Histology and Cytology·Tatsuo UshikiMasatsugu Shigeno
May 19, 1976·Human Genetics·J M Scheres, G F Merkx
Sep 1, 1984·Journal of Veterinary Pharmacology and Therapeutics·G E Burrows
Jul 1, 2017·Journal of Virology·Artur YakimovichJason Mercer
Jun 1, 1989·Journal of Molecular Evolution·G P Holmquist
Aug 13, 1976·Science·G P Holmquist, D E Comings

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