Mechanisms of complement resistance induced by non-lethal complement attack and by growth arrest

Immunology
K J MarchbankB Paul Morgan

Abstract

Non-lethal complement (C) attack on K562 cells has been shown to induce a transient resistance to lethal amounts of C. We have previously shown that incubation of K562 with phorbol 12-myristate 13-acetate (PMA) caused an increase in both CD59 expression and resistance to C killing and we were interested to examine whether non-lethal C attack caused a similar effect. We here demonstrate that expression of the C inhibitors decay-accelerating factor (DAF), membrane cofactor protein (MCP) and CD59 was unaltered on K562 after non-lethal C attack and that neutralization of these inhibitors with specific blocking antibodies did not reverse the induced resistance. In an effort to understand the mechanisms of resistance we searched for other conditions that might induce C resistance in K562 cells. Growth-arrested cells showed a similar degree of resistance to C killing. The levels of DAF and MCP on these cells were unaltered whereas expression of CD59 was markedly reduced. Non-lethal C attack on these growth-arrested cells induced a further increase in resistance to C killing, suggesting that the mechanisms of resistance were not identical. Indeed, resistance of non-lethally attacked cells was completely lost within 8 hr of attack where...Continue Reading

References

May 1, 1992·European Journal of Immunology·Y ReiterZ Fishelson
Feb 1, 1990·Experimental Cell Research·M Lalande
Jan 1, 1988·Annual Review of Biochemistry·H J Müller-Eberhard
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C L KoskiM L Shin
Jan 1, 1994·Springer Seminars in Immunopathology·B P Morgan, S Meri
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S W SherwoodR T Schimke

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Citations

Jul 12, 2007·Cell Division·Bradley S EllisonRobert J Boackle
Jun 24, 2010·Journal of Cardiothoracic Surgery·Chelsey L KingCamille L Hancock Friesen
Jan 29, 2000·The Journal of Eukaryotic Microbiology·D M ChuF Marciano-Cabral
Nov 4, 2000·Expert Opinion on Investigational Drugs·B R Lucchesi, E J Tanhehco
Dec 9, 2008·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Annalisa AngeliniGino Gerosa
Aug 7, 2007·Human Immunology·Amy H Tang, Jeffrey L Platt
Aug 12, 2014·Heart Views : the Official Journal of the Gulf Heart Association·F Fadhil Al-Amran, Murteza Shahkolahi
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A P DalmassoM S Abrahamsen

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