PMID: 11920758Mar 29, 2002Paper

Mechanisms of cytoprotective effect of amino acids on local toxicity caused by sodium laurate, a drug absorption enhancer, in intestinal epithelium

Journal of Pharmaceutical Sciences
Yoko EndoToshikiro Kimura

Abstract

Several amino acids, including L-glutamine (L-Gln), were found to protect the intestinal epithelial cells from the local toxicity caused by a drug absorption enhancer, sodium laurate (C12), in our previous study. To develop more efficient and safer formulations for enhancing drug absorption, the mechanisms of cytoprotection by amino acids were studied using rats and Caco-2 cells. Four amino acids, including L-Gln, could generally maintain the absorption-promoting action of C12, although taurine tended to attenuate it. Three amino acids, except for L-Gln, significantly suppressed the decrease in the transepithelial electrical resistance caused by C12. Quercetin, an inhibitor for biosynthesis of heat shock protein 70 (HSP70), masked only the protective effect of L-Gln in both rat large intestine and Caco-2 cells. Western blot analysis indicated clearly that HSP70 is induced extensively only by the addition of L-Gln in both rat large-intestinal cells and Caco-2 cells. C12 was found to increase the intracellular concentration of Ca(2+) ([Ca(2+)](i)) remarkably, and amino acids, especially L-arginine, L-methionine, and taurine, significantly attenuated the increase in [Ca(2+)](i) caused by C12. Furthermore, although C12 stimulated t...Continue Reading

References

Aug 15, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·M TakayamaI Tanimizu
Aug 1, 1992·Molecular and Cellular Biology·N HosokawaK Nagata
Jan 1, 1991·Journal of Cellular Physiology·M M Sanders, C Kon
Dec 1, 1988·Pharmaceutical Research·M TomitaS Awazu
Jan 1, 1987·Annual Review of Biochemistry·E Carafoli
Sep 1, 1985·JPEN. Journal of Parenteral and Enteral Nutrition·W W SoubaD W Wilmore
Apr 1, 1983·Physiological Reviews·Z Kovacevic, J D McGivan
Aug 1, 1994·Pharmaceutical Research·E S SwensonW Curatolo
Nov 28, 1996·European Journal of Pharmacology·Y Katsube, N Sperelakis
Feb 7, 1998·Pharmaceutical Research·P Artursson, R T Borchardt
Jun 13, 1998·Biochimica Et Biophysica Acta·F C Mooren, R K Kinne
Dec 5, 1998·Pharmacology & Therapeutics·J G Kiang, G C Tsokos
Sep 14, 1999·Biochemical Pharmacology·M PalmiK F Tipton
Mar 16, 2000·Molecular and Cellular Biochemistry·J G KiangG C Tsokos
Dec 18, 2001·Journal of Pharmaceutical Sciences·T YataT Kimura

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Citations

Apr 6, 2007·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Kazutaka Higaki
Aug 5, 2009·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Toshikiro Kimura
Sep 3, 2013·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Masateru Miyake
Jul 25, 2009·Journal of Pharmaceutical Sciences·Chie TakayamaToshikiro Kimura
Oct 5, 2007·International Journal of Pharmaceutics·Dipak S PisalSrinath Palakurthi
Apr 20, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yoshitsugu FujiokaToshikiro Kimura
Sep 3, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Masateru MiyakeToshikiro Kimura
Sep 16, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Masateru MiyakeToshikiro Kimura
Jan 18, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Masateru MiyakeToshikiro Kimura
Aug 25, 2016·Cytotechnology·B Martínez-VallespínJ Zentek
Aug 19, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Hong YuRamaswamy K Iyer
Mar 2, 2018·EFSA Journal·UNKNOWN EFSA Panel on Food Additives and Nutrient Sources added to Food (ANS)Claude Lambré

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