Mechanisms of decreased Vitamin D 1alpha-hydroxylase activity in prostate cancer cells

Molecular and Cellular Endocrinology
Jian Feng MaDonna M Peehl

Abstract

Vitamin D 1alpha-hydroxylase (1alpha(OH)ase), which converts the circulating prohormone 25-hydroxyvitamin-D(3) (25(OH)D(3)) to the active 1alpha-25-dihydroxyvitamin-D(3) (1,25(OH)(2)D(3)), is present in normal prostatic epithelium. However, prostate cancer cells, both primary cultured cells and cell lines, have greatly decreased activity of 1alpha(OH)ase and are therefore resistant to the tumor suppressor activity of circulating 25(OH)D(3). We quantitated 1alpha(OH)ase mRNA and protein levels to investigate mechanism(s) responsible for decreased 1alpha(OH)ase enzymatic activity in prostate cancer. Prostate cancer cell lines had low 1alpha(OH)ase mRNA levels. Primary prostate cell cultures derived from normal and cancer tissues had equivalent levels of 1alpha(OH)ase RNA and protein. Equivalent 1alpha(OH)ase protein levels were observed in prostate tissue sections containing normal and malignant cells. The protein levels of hsc70, whose homolog intracellular Vitamin D binding protein (IDBP-1) facilitates delivery of 25(OH)D(3) to 1alpha(OH)ase in monkey cells, were equivalent in the normal and cancer cells. Equivalent activity in normal and cancer cells of Vitamin D 24-hydroxylase, a mitochondrial enzyme that also uses 25(OH)D(3)...Continue Reading

References

Aug 1, 1989·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·D L TrumpV C Jordan
Jun 3, 1999·Proceedings of the Society for Experimental Biology and Medicine·S E Blutt, N L Weigel
Aug 24, 1999·Cancer Metastasis Reviews·G J Miller
Dec 10, 1999·Journal of the American Society of Nephrology : JASN·D ZehnderP M Stewart
Nov 15, 2000·Cancer Causes & Control : CCC·M H AhonenP Tuohimaa
Jul 27, 2001·Biochemical and Biophysical Research Communications·P BareisM Peterlik
Apr 17, 2002·The Journal of Biological Chemistry·Zhongjian XieDaniel D Bikle
Jul 12, 2002·The Urologic Clinics of North America·Badrinath R Konety, Robert H Getzenberg
Jul 26, 2002·The Journal of Steroid Biochemistry and Molecular Biology·Lyman W WhitlatchTai C Chen
Sep 5, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P W HochachkaP Kozlowski
Dec 31, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Tomasz M BeerW David Henner
Jan 10, 2003·Journal of Cellular Biochemistry·Tai C ChenMichael F Holick
Jan 10, 2003·Journal of Cellular Biochemistry·Aruna V KrishnanDavid Feldman
Feb 11, 2003·Journal of Cellular Biochemistry·Sylvia ChristakosAngela Porta

❮ Previous
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Citations

Jan 20, 2005·Journal of the National Cancer Institute·Scott M LippmanCharles A Coltman
Feb 9, 2007·The Journal of Steroid Biochemistry and Molecular Biology·T CordesM Friedrich
Oct 13, 2011·Advances in Preventive Medicine·Krishna Vanaja Donkena, Charles Y F Young
Aug 30, 2011·Best Practice & Research. Clinical Endocrinology & Metabolism·Helen L Henry
Mar 4, 2008·Molecular and Cellular Endocrinology·Paul H AndersonHoward A Morris
May 4, 2005·Cancer Causes & Control : CCC·Edward Giovannucci
Feb 8, 2008·Nutrition Reviews·Richard J Wood
May 16, 2009·International Journal of Cancer. Journal International Du Cancer·Rebecca GilbertRichard M Martin
Mar 14, 2007·The British Journal of Nutrition·Jean-Philippe BonjourPatrice Fardellone
Oct 7, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M van DrielJ P T M van Leeuwen
Aug 12, 2008·The American Journal of Clinical Nutrition·Anthony W Norman
Oct 2, 2019·The Journal of Steroid Biochemistry and Molecular Biology·Chuck C Blajszczak, Larisa Nonn
Oct 31, 2017·Endocrinology and Metabolism Clinics of North America·Moray J Campbell, Donald L Trump
Nov 17, 2011·Archives of Biochemistry and Biophysics·JoEllen Welsh

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