PMID: 7541525Apr 1, 1995Paper

Mechanisms of dopamine effects on Na-K-ATPase activity in Madin-Darby canine kidney (MDCK) epithelial cells

Pflügers Archiv : European journal of physiology
M ShahediC Sachs

Abstract

Dopamine decreases tubular sodium reabsorption, attributed in part to Na-K-ATPase inhibition in the proximal convoluted tubule (PCT). Because the final regulation of sodium excretion occurs in the collecting duct, where specific dopamine DA1 binding sites have been demonstrated, we examined the effects of dopamine, as well as of DA1 and DA2 receptor agonists on Na-K-ATPase activity and on the number of units in Madin-Darby canine kidney (MDCK) cells, which retain differentiated properties of the renal cortical collecting tubule epithelium. Dopamine (10(-5) M) inhibited pump activity (by 50%) and reduced the number of units. This effect was reproduced by the DA1 agonist SKF 38393, which inhibited pump activity in a dose- and time-dependent manner (maximum, 10(-5) M). The DA2 agonist quinpirole hydrochloride was without effect, either alone or in combination with SKF 38393. Inhibition of pump activity by dopamine was totally abolished by H7 (100 microM), an inhibitor of protein kinase (PK), but partially by 2',5'-dideoxy-adenosine (DDA) and H4, respective inhibitors of cAMP production and PKA, which suggests that the dopamine effect on Na-K-ATPase activity may be linked to activation of both PKC and PKA. In these cells, amiloride...Continue Reading

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Citations

Dec 5, 1998·Pharmacology & Therapeutics·P A JoseR A Felder
Nov 5, 1999·International Journal of Cardiology·A BabaS Ogawa
Mar 3, 2015·Current Behavioral Neuroscience Reports·E E M KnowlesD C Glahn
Mar 19, 2003·British Journal of Pharmacology·Pedro Gomes, Patrício Soares-da-Silva
Feb 11, 1998·Physiological Reviews·C MissaleM G Caron
Nov 4, 2004·Journal of Receptor and Signal Transduction Research·Kim A NeveHeather Trantham-Davidson
Aug 16, 2000·American Journal of Physiology. Cell Physiology·A G Therien, R Blostein
Jun 13, 2002·American Journal of Physiology. Renal Physiology·Pedro Gomes, P Soares-Da-Silva

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